Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69] (AB282196)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling Carboxypeptidase A with ab282196 at 1/100 dilution (5.0 µg/ml). Positive staining on human pancreas. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab282196 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69] (AB282196)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Carboxypeptidase A with ab282196 at 1/100 dilution (5.0 µg/ml). Negative control : no staining on human tonsil. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab282196 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69] (AB282196)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labelling Carboxypeptidase A with ab282196 at 1/100 dilution (5.0 µg/ml). Positive staining on rat pancreas. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab282196 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69] (AB282196)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labelling Carboxypeptidase A with ab282196 at 1/100 dilution (5.0 μg/ml / Green). Positive staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69] (AB282196)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat pancreas tissue labelling Carboxypeptidase A with ab282196 at 1/100 dilution (5.0 μg/ml / Green). Positive staining on rat pancreas is observed. The nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Carboxypeptidase A antibody [EPR24384-69] (AB282196)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labelling Carboxypeptidase A with ab282196 at 1/100 dilution (5.0 µg/ml). Positive staining on mouse pancreas. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab282196 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)