Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266]

6 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266] (ab314677)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Cardiac Troponin T with ab314677 at 1/100 (5.0 µg/ml). Negative control: no staining on human skeletal muscle. The section was incubated with ab314677 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266] (ab314677)

  Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Cardiac Troponin T with ab314677 at 1/100 (5.0 µg/ml). Cytoplasmic staining on rat cardiac myocytes. The section was incubated with ab314677 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266] (ab314677)

  Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Cardiac Troponin T with ab314677 at 1/100 (5.0 µg/ml). Cytoplasmic staining on mouse cardiac myocytes. The section was incubated with ab314677 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266] (ab314677)

  Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Cardiac Troponin T with ab314677 at 1/100 (5.0 µg/ml). Cytoplasmic staining on human cardiac myocytes. The section was incubated with ab314677 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266] (ab314677)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized H9c2(2-1) (rat cardiomyoblast) cells labelling Cardiac Troponin T with ab314677 at 1/500 dilution (0.1 µg)/ (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR20266] (ab314677)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (mouse atrial muscle cell) cells labelling Cardiac Troponin T with ab314677 at 1/500 dilution (0.1 µg)/(Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).