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AB198326

Alexa Fluor® 647 Anti-Cathepsin D antibody [EPR3057Y]

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(2 Publications)

Rabbit Recombinant Monoclonal Cathepsin D antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

CPSD, CTSD, Cathepsin D

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cathepsin D antibody [EPR3057Y] (AB198326)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cathepsin D antibody [EPR3057Y] (AB198326)

ab198326 staining Cathepsin D in MCF-7 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab198326 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Carrier free

    Anti-Cathepsin D antibody [EPR3057Y] - BSA and Azide free

  • Unconjugated

    Anti-Cathepsin D antibody [EPR3057Y]

  • 660 APC

    APC Anti-Cathepsin D antibody [EPR3057Y]

  • 421 Alexa Fluor® 405

    Alexa Fluor® 405 Anti-Cathepsin D antibody [EPR3057Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cathepsin D antibody [EPR3057Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Cathepsin D antibody [EPR3057Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Cathepsin D antibody [EPR3057Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Cathepsin D antibody [EPR3057Y]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Cathepsin D antibody [EPR3057Y]

  • HRP

    HRP Anti-Cathepsin D antibody [EPR3057Y]

  • 578 PE

    PE Anti-Cathepsin D antibody [EPR3057Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3057Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin D also known as CTSD is a protein with a mass of approximately 45 kDa. It functions as an aspartyl protease and is expressed in lysosomes across various tissues including the liver and kidneys. This enzyme acts by cleaving peptide bonds within proteins which is essential for protein degradation and turnover. Cathepsin D exists as precursor forms that become activated in the acidic environment of the lysosome. It plays a critical role in normal cellular processes by maintaining protein homeostasis.
Biological function summary

The enzymatic activity of Cathepsin D is important for cellular maintenance and apoptosis. This protease does not act within larger protein complexes but contributes to the degradation of extracellular and intracellular proteins. It mediates processes like antigen processing where it deconstructs proteins into peptides that are presented on major histocompatibility complex (MHC) molecules. ELISA tests can quantify its expression levels sometimes termed as CTSD activity in various biological samples offering insights into its role within cellular environments.

Pathways

Cathepsin D involvement includes the lysosomal degradation pathway and the apoptotic signaling pathway. In the lysosomal degradation pathway Cathepsin D breaks down proteins and peptides a process important for cellular recycling and energy release. It interacts with other lysosomal enzymes such as Cathepsin B in this pathway ensuring comprehensive breakdown of cellular waste. The apoptotic signaling pathway involves the regulation of programmed cell death where Cathepsin D can influence the activation of downstream proteins like Bcl-2 and Bax which control cell survival.

The overexpression of Cathepsin D links to breast cancer and Alzheimer's disease. In breast cancer increased Cathepsin D expression correlates with tumor progression and metastasis influencing tumor behavior through interactions with other proteins involved in cell proliferation. Alzheimer's disease features the involvement of Cathepsin D in the breakdown of amyloid precursor protein which relates to amyloid beta plaque accumulation. The abnormal activity of Cathepsin D in these disorders makes it a potential target for therapeutic antibodies such as CTSD antibodies which aim to regulate its function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed : 27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
See full target information CTSD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 58:2495-2509.e6 PubMed37683630

2023

Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wen Teo,Ho Him Wong,Tiaan Heunis,Viktoriya Stancheva,Asmaa Hachim,Huibin Lv,Lewis Siu,Julian Ho,Yun Lan,Chris Ka Pun Mok,Rachel Ulferts,Sumana Sanyal

EMBO reports 24:e57499 PubMed37401859

2023

ER-mitochondria contacts and cholesterol metabolism are disrupted by disease-associated tau protein.

Applications

Unspecified application

Species

Unspecified reactive species

Leonora Szabo,Nadia Cummins,Paolo Paganetti,Alex Odermatt,Andreas Papassotiropoulos,Celeste Karch,Jürgen Götz,Anne Eckert,Amandine Grimm
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com