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AB307404

Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84]

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Rabbit Recombinant Monoclonal CCL18 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

AMAC1, DCCK1, MIP4, PARC, SCYA18, CCL18, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1, CC chemokine PARC, Dendritic cell chemokine 1, Macrophage inflammatory protein 4, Pulmonary and activation-regulated chemokine, Small-inducible cytokine A18, AMAC-1, DC-CK1, MIP-4

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84] (AB307404)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84] (AB307404)

Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling CCL18 with ab307404 at 1/50 (10.0 ug/ml) dilution. Positive staining on human colon carcinoma. The section was incubated with ab307404 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84] (AB307404)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84] (AB307404)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCL18 with ab307404 at 1/50 (10.0 ug/ml) dilution. Positive staining on human tonsil. The section was incubated with ab307404 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84] (AB307404)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CCL18 antibody [EPR24781-84] (AB307404)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling CCL18 with ab307404 at 1/50 (10.0 ug/ml) followed by a at dilution. Negative control : no staining on human skeletal muscle.The section was incubated with ab307404 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24781-84

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL18 also known as pulmonary and activation-regulated chemokine (PARC) is a chemokine protein with a molecular weight of about 8.8 kDa. It is mainly expressed in the lung but also found in lymph nodes and placenta. CCL18 is secreted by activated macrophages and dendritic cells. As a chemokine it plays a role in immune system signaling where it attracts immune cells like T lymphocytes. Researchers utilize CCL18 ELISA kits to accurately measure its levels in biological samples providing insight into its functions.
Biological function summary

CCL18 plays an important role in modulating immune responses and recruiting immune cells to sites of inflammation. It is not part of a larger complex but works closely with other components of the immune system. CCL18 influences the activity of monocytes and the differentiation of T cells which are essential for mounting effective defense against pathogens. In inflammatory conditions increased CCL18 levels suggest its importance in controlling the magnitude and duration of immune responses.

Pathways

CCL18 is involved in the immune response and inflammatory pathways. It works particularly in chemokine signaling pathways and shares functional relations with proteins like CCR3 and CCR6 which act as receptors for various chemokines. The binding of CCL18 to these receptors on targeted cells facilitates the chemotaxis of immune cells steering the immune response to appropriate areas in the body where they are needed.

CCL18 has known associations with pulmonary fibrosis and various cancers. In pulmonary fibrosis CCL18 levels correlate with disease severity and progression indicating its potential role as a biomarker. The protein interacts with other signaling molecules including collagen and matrix metalloproteinases driving the fibrotic process. In cancers especially breast cancer high CCL18 expression often corresponds to tumor progression and metastasis. These interactions highlight its relevance not only in disease progression but also as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
See full target information CCL18
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com