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AB270659

Alexa Fluor® 647 Anti-CCR3 antibody [5E8]

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(1 Publication)

Mouse Monoclonal CCR3 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human CCR3.

View Alternative Names

CD193, CMKBR3, CCR3, C-C chemokine receptor type 3, C C CKR3, C-C CKR-3, CC-CKR-3, CCR-3, CKR 3, CKR3, Eosinophil eotaxin receptor

2 Images
Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [5E8] (AB270659)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [5E8] (AB270659)

Flow cytometry analysis of human basophil granulocytes (red) and lymphocyte (black) cells labeling CCR3 using ab270659. Concentration used was 4 μl reagent/100μl peripheral whole blood.

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [5E8] (AB270659)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [5E8] (AB270659)

Flow cytometry analysis of human peripheral whole blood cells labeling CCR3 using ab270659. The concentration used was 4 μl reagent/ 100μl of peripheral whole blood.

  • 578 PE

    PE Anti-CCR3 antibody [5E8]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CCR3 antibody [5E8]

  • 660 APC

    APC Anti-CCR3 antibody [5E8]

  • 519 FITC

    FITC Anti-CCR3 antibody [5E8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E8

Isotype

IgG2b

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human CCR3. The exact immunogen used to generate this antibody is proprietary information.

P51677

Epitope

extracellular

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR3 also known as C-C chemokine receptor type 3 is a protein that acts as a receptor for chemokines. The molecular weight of CCR3 is approximately 43 kDa. This protein is predominantly expressed in eosinophils and basophils and is also found in specific subsets of T cells. The expression of CCR3 in these cells plays an important role in directing their migration and activity. This receptor is part of the G protein-coupled receptor family which transduce signals across the cell membrane.
Biological function summary

CCR3 facilitates chemokine-directed migration of white blood cells an essential function for immune responses. It binds to several chemokines like eotaxin eotaxin-2 and RANTES which mediates the recruitment of eosinophils and other cells to sites of inflammation. CCR3 does not form part of a protein complex instead it interacts directly with its chemokine ligands. This activity enhances the body's ability to respond to allergic reactions and inflammatory processes.

Pathways

CCR3 plays a role in the signaling pathways that orchestrate immune cell trafficking and activation. It is involved in the chemokine signaling pathway which regulates cell movement and positioning and the calcium mobilization pathway through its ligand interaction. CCR3 along with other receptors like CCR5 and CXCR4 facilitates the communication between immune cells aiding in directed cell movement towards the inflammation site.

CCR3 is notably involved in allergic disorders and asthma. Its expression influences the recruitment of eosinophils which are key players in these conditions. This receptor is linked to heightened inflammatory responses leading to tissue damage in chronic allergic diseases. The interaction of CCR3 with chemokines like eotaxin is significant in the pathophysiology of these diseases making it a potential target for therapeutic intervention to mitigate symptoms of chronic allergic responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15 (PubMed : 7622448, PubMed : 8642344, PubMed : 8676064). Subsequently transduces a signal by increasing the intracellular calcium ions level (PubMed : 8676064). In addition acts as a possible functional receptor for NARS1 (PubMed : 30171954).. (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
See full target information CCR3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:904969 PubMed35978822

2022

Cyclic adenosine monophosphate/phosphodiesterase 4 pathway associated with immune infiltration and PD-L1 expression in lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Tong,Minjie Shan,Wen Zou,XianLing Liu,Dean W Felsher,Jingjing Wang
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