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AB323785

Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30]

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Rabbit Recombinant Monoclonal CCR3 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD193, Cmkbr1l2, Cmkbr3, Ccr3, Probable C-C chemokine receptor type 3, C-C CKR-3, CC-CKR-3, CCR-3, CCR3, CKR3, Macrophage inflammatory protein 1-alpha receptor-like 2, MIP-1 alpha RL2

4 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CCR3 with ab323785 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in J774A.1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : A20.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/ab195887 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)

Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with ab323785 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were co-stained with anti-CD3 cojugated to Alexa Fluor®488.
Gated on viable cells.

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)

Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with ab323785 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.
Gated on viable cells.

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] (AB323785)

Flow cytometric analysis of A20 (mouse reticulum sarcoma B lymphocyte, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage, Right) cells labelling CCR3 with ab323785 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Gated on viable cells.
Low expression : A20.

  • 578 PE

    PE Anti-CCR3 antibody [EPR27419-30]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CCR3 antibody [EPR27419-30]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CCR3 antibody [EPR27419-30]

  • Unconjugated

    Anti-CCR3 antibody [EPR27419-30]

  • Carrier free

    Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27419-30

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR3 also known as C-C chemokine receptor type 3 is a protein that acts as a receptor for chemokines. The molecular weight of CCR3 is approximately 43 kDa. This protein is predominantly expressed in eosinophils and basophils and is also found in specific subsets of T cells. The expression of CCR3 in these cells plays an important role in directing their migration and activity. This receptor is part of the G protein-coupled receptor family which transduce signals across the cell membrane.
Biological function summary

CCR3 facilitates chemokine-directed migration of white blood cells an essential function for immune responses. It binds to several chemokines like eotaxin eotaxin-2 and RANTES which mediates the recruitment of eosinophils and other cells to sites of inflammation. CCR3 does not form part of a protein complex instead it interacts directly with its chemokine ligands. This activity enhances the body's ability to respond to allergic reactions and inflammatory processes.

Pathways

CCR3 plays a role in the signaling pathways that orchestrate immune cell trafficking and activation. It is involved in the chemokine signaling pathway which regulates cell movement and positioning and the calcium mobilization pathway through its ligand interaction. CCR3 along with other receptors like CCR5 and CXCR4 facilitates the communication between immune cells aiding in directed cell movement towards the inflammation site.

CCR3 is notably involved in allergic disorders and asthma. Its expression influences the recruitment of eosinophils which are key players in these conditions. This receptor is linked to heightened inflammatory responses leading to tissue damage in chronic allergic diseases. The interaction of CCR3 with chemokines like eotaxin is significant in the pathophysiology of these diseases making it a potential target for therapeutic intervention to mitigate symptoms of chronic allergic responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15. Subsequently transduces a signal by increasing the intracellular calcium ions level. In addition acts as a possible functional receptor for NARS1.
See full target information Ccr3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com