Alexa Fluor® 647 Anti-CD10 antibody [EPR22867-118]

5 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD10 antibody [EPR22867-118] (ab309082)

  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD10 with ab309082 at 1/100 (5.0 ug/ml).
  
  Membranous staining on rat kidney.
  
  The section was incubated with ab309082 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD10 antibody [EPR22867-118] (ab309082)

  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD10 with ab309082 at 1/100 (5.0 ug/ml).
  
  Membranous staining on mouse kidney.
  
  The section was incubated with ab309082 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD10 antibody [EPR22867-118] (ab309082)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CD10 with ab309082 at 1/100 (5.0 ug/ml).
  
  Negative control: no staining on human skeletal muscle.
  
  The section was incubated with ab309082 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD10 antibody [EPR22867-118] (ab309082)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab309082 at 1/100 (5.0 ug/ml).
  
  Membranous staining on human kidney.
  
  The section was incubated with ab309082 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD10 antibody [EPR22867-118] (ab309082)

  Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized Ramos (human Burkitt's lymphoma B lymphocyte) cells labelling CD10 with ab309082 at 1/50 (10.0 ug/ml) dilution.
  
  Confocal image showing membranous staining in Ramos cells. Negative control: HT-29 (PMID:19828468). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).