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Rabbit Recombinant Monoclonal ITAL antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (AB307264), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (AB307264), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (AB307264), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (AB307264), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Version

Target data

Function

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed:11812992, PubMed:15528364). Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed:29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed:15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:11812992). Acts as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed:38195629). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ITAL antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1285Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD11a also known as ITGAL and LFA-1 alpha chain or integrin alpha L is a protein that functions as an important component of the immune system. It has a molecular mass of approximately 180 kDa. CD11a is expressed predominantly in leukocytes enabling these immune cells to adhere and interact with other cells. This adhesive property is especially critical in the trafficking of leukocytes to sites of inflammation and infection. CD11a pairs with CD18 to form the leukocyte function-associated antigen-1 (LFA-1) complex which plays a significant role in cell signaling and immune responses.

Biological function summary

This protein enables leukocyte adhesion and transendothelial migration which are essential for the immune surveillance process. CD11a is a part of the integrin family known for regulating cell adhesion and interactions with the extracellular matrix. By forming a heterodimer with CD18 it mediates tight adhesion between immune cells and endothelial cells. This interaction aids in the immune response by enhancing the activation and movement of T cells and other leukocytes through the vascular endothelium into tissues.

Pathways

The protein participates in the immune cell trafficking pathway and the integrin signaling pathway. In these pathways CD11a contributes to the modulation of immune responses and cell movement. The interaction of CD11a with intercellular adhesion molecule 1 (ICAM-1) and other proteins within these pathways emphasizes its role in the firm adhesion of leukocytes during inflammation and infection ultimately facilitating leukocyte extravasation and tissue infiltration.

Associated diseases and disorders

CD11a is highly relevant to autoimmune diseases such as psoriasis and multiple sclerosis. For instance in psoriasis the overactive immune response involves overexpression of integrins including CD11a leading to inflammation. The protein Efalizumab a monoclonal antibody against CD11a has been used in the treatment of psoriasis to reduce leukocyte adhesion and activation. Such relationships highlight CD11a's critical role in both normal immune function and pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labelling CD11a with ab307264 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor? 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264)

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcin tissue labeling CD11a with ab307264 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on immune cells of human thyroid carcinoma.The section was incubated with ab307264 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD11a with ab307264 at 1/100 (5.0 ug/ml) followed by a at dilution. Positive staining on human tonsil.The section was incubated with ab307264 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD11a antibody [EP1285Y] (ab307264)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling CD11a with ab307264 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor? 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A

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Product protocols

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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