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AB216524

Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42]

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(3 Publications)

Mouse Monoclonal CD11b + CD11c antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Rat samples. Cited in 3 publications.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

2 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (AB216524)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (AB216524)

IHC image of CD11b + CD11c staining in a section of frozen normal rat brain.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab216524 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (AB216524)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (AB216524)

IHC image of CD11b + CD11c staining in a section of frozen normal rat spleen.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab216524 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b + CD11c antibody [OX42]

  • Biotin

    Biotin Anti-CD11b + CD11c antibody [OX42]

  • Unconjugated

    Anti-CD11b + CD11c antibody [OX42]

  • Carrier free

    Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX42

Isotype

IgG2a

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b and CD11c also known as integrins αM and αX respectively are important proteins in the immune system. CD11b with a molecular mass of approximately 165 kDa and CD11c around 150 kDa form integrin complexes with CD18. These proteins are expressed on various immune cells including macrophages monocytes granulocytes and dendritic cells. Their expression can also be detected in a subset of B and T cells. The antibody against CD11b also termed OX-42 is often used in research to identify immune cells that express this surface protein.
Biological function summary

Integrins αM (CD11b) and αX (CD11c) facilitate key processes such as adhesion and migration of leukocytes. Both CD11b and CD11c pair with CD18 to form the Mac-1 (Macrophage-1 antigen also known as CR3) and p15095 complexes respectively. These complexes participate in both innate and adaptive immune responses by binding to various ligands like ICAM-1 and fibrinogen. CD11b/CD18 (Mac-1) additionally plays an essential role in phagocytosis degranulation and respiratory burst important activities for pathogen clearance.

Pathways

These integrins engage in critical signaling pathways including the NF-kappaB and MAPK pathways. They regulate the inflammatory response and cell survival. CD11b interacts closely with other proteins like ICAM-1 and C3b which are involved in these pathways. CD11c also contributes to similar pathways although it plays a more specialized role in dendritic cells impacting antigen presentation and immune regulation.

CD11b and CD11c have associations with conditions like autoimmune diseases and chronic inflammatory disorders. Both proteins show altered expression levels in diseases such as rheumatoid arthritis and systemic lupus erythematosus. For instance in these diseases the Mac-1 complex (CD11b/CD18) frequently exhibits dysregulation affecting leukocyte adhesion and migration. The expression of CD11c is modified in certain leukemias and lymphomas where it correlates with disease progression and prognosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM

Additional targets

Integrin subunit alpha X
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Purinergic signalling 21:565-576 PubMed40560521

2025

Electroacupuncture alleviates capsaicin-induced rectal visceral pain in rats via inhibiting TRPV1 expression by blocking the P2X4R-activated p38 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yahong Xue,Qinbing Zhu,Jing Zhang,Xiaofeng Wang

Clinical cancer research : an official journal of the American Association for Cancer Research 31:746-755 PubMed39704655

2024

Smoking-Associated Carcinogen-Induced Inflammation Promotes Lung Carcinogenesis via IRAK4 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ritesh K Aggarwal,Simone Sidoli,Jingli Wang,Srabani Sahu,Rahul Sanawar,Varun Gupta,Srinivas Aluri,Vineeth Sukrithan,Charan T R Vegivinti,Phaedon D Zavras,Divij Verma,Shanisha Gordon-Mitchell,Beamon Agarwal,Tanya Verma,Daniel T Starczynowski,Ulrich G Steidl,Aditi Shastri,Balazs Halmos,Lindsay M LaFave,Haiying Cheng,Amit Verma,Yiyu Zou

Frontiers in aging neuroscience 13:798335 PubMed34966272

2021

Lipocalin-2 Regulates Hippocampal Microglial Activation in Poststroke Depression.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wei,Yupeng Du,Yirui Xie,Xiaopeng Yu,Hui Chen,Yunqing Qiu
View all publications
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