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Mouse Monoclonal CDK11B antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (AB216524), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (AB216524), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-Fr
Human
Predicted
Rat
Tested

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Version

Target data

Function

Plays multiple roles in cell cycle progression, cytokinesis and apoptosis. Involved in pre-mRNA splicing in a kinase activity-dependent manner. May act as a negative regulator of normal cell cycle progression.

Additional Targets

Integrin subunit alpha X

Alternative names

Cdc2l1, Cdk11, Cdk11b, Cyclin-dependent kinase 11B, Cell division cycle 2-like protein kinase 1, Cell division protein kinase 11, Cyclin-dependent kinase 11, Galactosyltransferase-associated protein kinase p58/GTA, PITSLRE serine/threonine-protein kinase CDC2L1

Recommended products

Mouse Monoclonal CDK11B antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-Fr and reacts with Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG2a
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
OX42
Purification technique
Affinity purification Protein G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD11b and CD11c also known as integrins αM and αX respectively are important proteins in the immune system. CD11b with a molecular mass of approximately 165 kDa and CD11c around 150 kDa form integrin complexes with CD18. These proteins are expressed on various immune cells including macrophages monocytes granulocytes and dendritic cells. Their expression can also be detected in a subset of B and T cells. The antibody against CD11b also termed OX-42 is often used in research to identify immune cells that express this surface protein.

Biological function summary

Integrins αM (CD11b) and αX (CD11c) facilitate key processes such as adhesion and migration of leukocytes. Both CD11b and CD11c pair with CD18 to form the Mac-1 (Macrophage-1 antigen also known as CR3) and p15095 complexes respectively. These complexes participate in both innate and adaptive immune responses by binding to various ligands like ICAM-1 and fibrinogen. CD11b/CD18 (Mac-1) additionally plays an essential role in phagocytosis degranulation and respiratory burst important activities for pathogen clearance.

Pathways

These integrins engage in critical signaling pathways including the NF-kappaB and MAPK pathways. They regulate the inflammatory response and cell survival. CD11b interacts closely with other proteins like ICAM-1 and C3b which are involved in these pathways. CD11c also contributes to similar pathways although it plays a more specialized role in dendritic cells impacting antigen presentation and immune regulation.

Associated diseases and disorders

CD11b and CD11c have associations with conditions like autoimmune diseases and chronic inflammatory disorders. Both proteins show altered expression levels in diseases such as rheumatoid arthritis and systemic lupus erythematosus. For instance in these diseases the Mac-1 complex (CD11b/CD18) frequently exhibits dysregulation affecting leukocyte adhesion and migration. The expression of CD11c is modified in certain leukemias and lymphomas where it correlates with disease progression and prognosis.

Product promise

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2 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (ab216524), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (ab216524)

    IHC image of CD11b + CD11c staining in a section of frozen normal rat brain.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab216524 at 1/100 dilution (shown in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (ab216524), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (ab216524)

    IHC image of CD11b + CD11c staining in a section of frozen normal rat spleen.

    The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab216524 at 1/100 dilution (shown in red) and counterstained using Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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