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AB252127

Alexa Fluor® 647 Anti-CD133 antibody [EPR20980-104]

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(2 Publications)

Rabbit Recombinant Monoclonal CD133 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD133, PROML1, MSTP061, PROM1, Prominin-1, Antigen AC133, Prominin-like protein 1

1 Images
Flow Cytometry - Alexa Fluor® 647 Anti-CD133 antibody [EPR20980-104] (AB252127)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-CD133 antibody [EPR20980-104] (AB252127)

Overlay histogram showing left NCCIT positive cells and right negative HEK-293 cells stained with ab252127 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab252127) (1x 106 in 100μl at 0.2 μg/ml (1/2500)) for 30 min on ice.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

  • 578 PE

    PE Anti-CD133 antibody [EPR20980-104]

  • 660 APC

    APC Anti-CD133 antibody [EPR20980-104]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD133 antibody [EPR20980-104]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD133 antibody [EPR20980-104]

  • Unconjugated

    Anti-CD133 antibody [EPR20980-104]

  • Carrier free

    Anti-CD133 antibody [EPR20980-104] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20980-104

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD133 also known as Prominin-1 is a transmembrane glycoprotein with a molecular weight of approximately 120 kDa. This protein stands out due to its expression in various stem and progenitor cells including those of the neural hematopoietic and epithelial lineages. CD133 is recognized for its distinct five transmembrane domains and two large extracellular loops. Researchers commonly use CD133 as a cell surface marker to identify stem and progenitor cells. The significance of CD133 extends to immunohistochemistry (IHC) and Western blot applications where it is a popular target for detection and analysis.
Biological function summary

CD133 is involved in maintaining the cellular architecture and signaling processes. It plays a role in cellular differentiation and proliferation while acting as a part of larger protein complexes on the cell surface. This protein is notable as it contributes significantly to the regulation of stem cell properties supporting the growth and repair in various tissues. Anti-CD133 antibodies are valuable tools in studying these functions due to their specificity in targeting this marker.

Pathways

CD133 participates in cellular signaling and transport pathways vital for intercellular communication and homeostasis. It interacts within pathways related to cell polarity and asymmetric division both essential for tissue development and maintenance. CD133 is associated with proteins like CD133/1 and CD133/2 which further highlights its involvement in these complex biological networks and on pathways such as the Wnt signaling pathway which regulates gene expression associated with cell proliferation and differentiation.

Several studies indicate a strong relationship between CD133 expression and certain cancers such as glioblastoma and colorectal cancer. In these cases CD133 serves not only as a marker but also as an indicator of the presence of cancer stem cells which are capable of tumor initiation and maintenance. Furthermore researchers have identified connections between CD133 and other proteins such as c-Met (cMab) within these pathological contexts suggesting interactions that could contribute to disease progression and offering potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in cell differentiation, proliferation and apoptosis (PubMed : 24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed : 20818439).
See full target information PROM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of bone oncology 50:100655 PubMed39850453

2025

Using β-Elemene to reduce stemness and drug resistance in osteosarcoma: A focus on the AKT/FOXO1 signaling pathway and immune modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Shaochun Zhang,Zhijie Xing,Jing Ke

American journal of cancer research 13:1999-2012 PubMed37293147

2023

Exosomal transfer of miR-548aq-3p confers cisplatin resistance MED12 downregulation in epithelial ovarian cancer.

Applications

Flow Cyt

Species

Human

Yanlong Shi,Yuwei Zou,Yu Guo,Yulin Liu,Qiang Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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