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AB199200

Alexa Fluor® 647 Anti-CD151 antibody [11G5a]

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(1 Publication)

Mouse Monoclonal CD151 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD151, TSPAN24, CD151 antigen, GP27, Membrane glycoprotein SFA-1, Platelet-endothelial tetraspan antigen 3, Tetraspanin-24, PETA-3, Tspan-24

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD151 antibody [11G5a] (AB199200)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD151 antibody [11G5a] (AB199200)

ab199200 staining CD151 in HT1080 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab199200 at 2µg/ml (shown in magenta) and ab195883, Rat monoclonal [YOL1/34] to alpha Tubulin 1 - Microtubule Marker (Alexa Fluor® 488), pre-adsorbed at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Unconjugated

    Anti-CD151 antibody [11G5a]

  • 519 FITC

    FITC Anti-CD151 antibody [11G5a]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

11G5a

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

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Alternative Products

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD151 also known as PETA-3 or SFA-1 is a member of the tetraspanin family which are small membrane proteins with molecular mass of about 28 kDa. This protein is commonly expressed on various cells such as epithelial cells endothelial cells and platelets. CD151 is known to interact with integrins especially integrin α3β1 and α6β1 playing significant roles in cell adhesion and migration. It is mostly found in areas where cell-to-cell contact is vital for maintaining tissue integrity and cellular communication.
Biological function summary

CD151 engages in the stabilization of integrin complexes which facilitates signal transduction pathways that influence cellular behaviors like proliferation differentiation and survival. It does not function alone; CD151 is part of a tetraspanin-enriched microdomain (TEM) forming complexes that include proteins such as CD9 CD63 and other integrins. These complexes bring together structural and signaling molecules on the cell membrane creating platforms for efficient signal relay.

Pathways

CD151 plays a role in pathways involving cell adhesion and tissue repair. Notably it is involved in the integrin signaling pathway which is essential for cell-matrix interactions in tissues that require adhesion for repair or restructuring. CD151 interacts with proteins like PI3K and Akt within these pathways which are central to the signaling cascades regulating cellular responses to the extracellular environment.

CD151 participation has been noted in cancer progression and metastasis. It links to the dysregulation observed in integrin functions such as those of integrin α3β1 which directly influences tumor cell invasion and metastasis in cancers like breast and prostate cancer. CD151 also exhibits connections with renal pathologies including Alport syndrome where the lack of functional basement membranes affects kidney function and it interacts with proteins like collagen IV in the disorder's progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Plays a role in various cellular and molecular mechanism through its association with both integrin and non-integrin proteins. These interactions facilitate critical cellular functions, including cell-to-cell communication, wound healing, platelet aggregation, trafficking, cell motility, and angiogenesis (PubMed : 17045834, PubMed : 24723389, PubMed : 31488507). Via interaction with JAM-A/F11R and integrin ITGA3 : ITGB1, promotes the recruitment of signaling molecules such as RAC1, CDC42 and RhoGTPases to facilitate the polarization of epithelial cells and the reorganization of the actin cytoskeleton, which are critical steps in cell migration process (PubMed : 22843693, PubMed : 35067832). Regulates the glycosylation pattern of ITGA3 : ITGB1 thereby modulating its activity (PubMed : 18852263). Plays an essential role in the maintenance of central laminin-binding integrin ITGA6 : ITGB4-containing adhesion complexes (PubMed : 31488507). Essential for the proper assembly of the glomerular and tubular basement membranes in kidney (PubMed : 15265795). Contributes to T-cell activation by modulating integrin signaling leading to activation of downstream targets PTK2 and MAPK1/MAPK3 (PubMed : 24723389).. (Microbial infection) Plays a role in human papillomavirus 16/HPV-16 endocytosis upon binding to cell surface receptor.. (Microbial infection) Plays a role in human cytomegalovirus entry into host cell by contributing to entry receptor binding, membrane fusion, or release of the capsid.
See full target information CD151
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pediatrics international : official journal of the Japan Pediatric Society 64:e15159 PubMed35727889

2022

The histone deacetylase inhibitor OBP-801 has in vitro/in vivo anti-neuroblastoma activity.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Kaneda,Tomoko Iehara,Ken Kikuchi,Yohei Sugimoto,Norio Nakagawa,Shigeki Yagyu,Mitsuru Miyachi,Eiichi Konishi,Toshiyuki Sakai,Hajime Hosoi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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