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Mouse Monoclonal KLRB1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human, Primates samples. Immunogen corresponding to Cell preparation containing KLRB1 protein.

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Images

Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (AB270661), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (AB270661), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (AB270661), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Cell preparation containing KLRB1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q12918

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested
Primates
Expected

Tested
Tested

Species

Human

Dilution info

4 µL for 106 Cells

Notes

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10^6 cells in suspension.

Expected
Expected

Species

Primates

Dilution info

4 µL for 106 Cells

Notes

The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10^6 cells in suspension.

Target data

Function

Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

Alternative names

Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, HNKR-P1a, Natural killer cell surface protein P1A, NKR-P1A, KLRB1, NKRP1A, CLEC5B

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Mouse Monoclonal KLRB1 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human, Primates samples. Immunogen corresponding to Cell preparation containing KLRB1 protein.

Alternative names

Killer cell lectin-like receptor subfamily B member 1, C-type lectin domain family 5 member B, HNKR-P1a, Natural killer cell surface protein P1A, NKR-P1A, KLRB1, NKRP1A, CLEC5B

Key facts

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Cell preparation containing KLRB1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q12918
Clone number

HP-3G10

Purification technique

Size-exclusion chromatography

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

Activity summary

CD161 also known as NKRP1A or KLRB1 is a type II transmembrane protein expressed primarily on natural killer (NK) cells and subsets of T cells. Weighing approximately 30-40 kDa CD161 functions as a C-type lectin receptor involved in the immune response. Its expression can be detected in various tissues including the liver and spleen. Researchers utilize tools such as mouse CD161 PE as well as CD161 APC antibodies for study of its cell surface expression and role in immune modulation.

Biological function summary

CD161 plays a role in immune system regulation by mediating NK cell and T cell activity. It does not operate as part of a complex but interacts with other cellular receptors to modulate immune responses. For example it serves as an inhibitory receptor often through engagement with its ligand LLT1 to temper immune responses and prevent overactivation. This balance is key to maintaining immune homeostasis and facilitates pathogen clearance while avoiding excessive tissue damage.

Pathways

CD161 is involved in the immune checkpoint pathways and is linked to inflammatory response signaling. These pathways play an important role in the immune system's ability to discern self from non-self determining the intensity and character of the body's defense mechanisms. Other proteins such as CTLA-4 and PD-1 also function within these pathways contributing to the regulation of immune cell activity and preventing autoimmunity.

Associated diseases and disorders

CD161 is associated with inflammatory diseases and cancer. Its involvement in immune checkpoints implicates it in conditions such as autoimmune diseases where it may fail to properly regulate immune activity including multiple sclerosis. In cancer CD161 expression on T cells has been examined in the context of its inhibitory role potentially modulating the immune system's ability to target tumor cells effectively. In these scenarios its interaction with LLT1 and other immune checkpoint proteins influences the disease course and therapeutic outcomes.

Product promise

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3 product images

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (ab270661), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (ab270661)

    Flow cytometry analysis of human peripheral whole blood labeling CD161 positive lymphocytes using ab270661 (Red) at 4 μl reagent in 100 μl of peripheral whole blood. Control: neutrophil granulocytes (Black).

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (ab270661), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (ab270661)

    Flow cytometry analysis of human lymphocytes labeling CD-161 using ab270661 at 4 μl reagent in 100 μl of peripheral whole blood. Control: anti-human CD4 (MEM-241) Pacific Blue™ antibody at 4 μl reagent in 100 μl of peripheral whole blood).

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (ab270661), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD161 antibody [HP-3G10] (ab270661)

    Flow cytometry analysis of human peripheral whole blood labeling CD161 using ab270661 at 4 μl reagent in 100 μl of peripheral whole blood.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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