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AB270662

Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104]

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(1 Publication)

Mouse Monoclonal CD200 / OX2 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD200.

View Alternative Names

CD200, MOX1, MOX2, My033, OX-2 membrane glycoprotein

3 Images
Flow Cytometry - Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104] (AB270662)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104] (AB270662)

Flow cytometric analysis of human lymphocytes labeling CD200 with ab270662 at 4μl reagent /100 μl of peripheral whole blood and an anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104] (AB270662)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104] (AB270662)

Separation of human CD200 positive CD3 negative lymphocytes (Red) from CD200 negative T-cells (black-dashed) in flow cytometric analysis labeling CD200 using ab270662. (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104] (AB270662)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 647 Anti-CD200 / OX2 antibody [OX-104] (AB270662)

Flow cytometric analysis of human peripheral whole blood labeling CD200 with ab270662 at 4 μl reagent / 100 μl of peripheral whole blood.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-104

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CD200. The exact immunogen used to generate this antibody is proprietary information.

P41217

Epitope

extracellular

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

AB150077

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  • 5
  • 20
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Reactivity data

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified from Tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD200 also known as OX2 is a cell surface glycoprotein with a molecular mass of approximately 43 kDa. It plays a mechanical role in immune response regulation. CD200 expression occurs on several cell types such as thymocytes neurons vascular endothelium and splenocytes including cynomolgus monkey splenocytes. This protein generally modulates interactions between cells sending inhibitory signals through its receptor CD200R found on myeloid cells and certain lymphoid cells.
Biological function summary

CD200 regulates the immune system by inhibiting inflammatory responses and maintaining immune homeostasis. It does not act as part of a significant multi-protein complex but directly interacts with CD200R. This interaction promotes an anti-inflammatory state and is important in preventing autoimmune diseases and excessive tissue damage. The expression of CD200 is also an indicator of immune tolerance in various tissues.

Pathways

CD200 is involved in immune regulatory pathways. It plays a pivotal role in the suppression of immune responses through the CD200-CD200R signaling pathway. This pathway is vital for dampening inflammatory processes related to autoimmune reactions and is consequently linked to the maintenance of tissue homeostasis. Proteins related to CD200 through these pathways include CD200R and other molecules involved in immune checkpoint pathways.

CD200 expression correlates with several conditions including cancer and autoimmune diseases. The overexpression of CD200 in certain tumors promotes immune evasion thereby favoring tumor progression. Conversely reduced CD200 levels are associated with autoimmune disorders where a hyperactive immune system attacks self-tissues. Through these disease contexts CD200 operates alongside proteins such as CD200R and OX90 impacting disease progression and immune system modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
See full target information CD200
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:904969 PubMed35978822

2022

Cyclic adenosine monophosphate/phosphodiesterase 4 pathway associated with immune infiltration and PD-L1 expression in lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Tong,Minjie Shan,Wen Zou,XianLing Liu,Dean W Felsher,Jingjing Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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