Alexa Fluor® 647 Anti-CD21 antibody [EP3093]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD21 antibody [EP3093] (AB202693)

ab202693 staining CD21 in Raji cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab202693 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in Raji cells fixed with 80% methanol (5 min).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD21 antibody [EP3093] (AB202693)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD21 with ab202693 at 1/100 (5.0 μg/ml) dilution.

Positive staining on human tonsil.

The section was incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab202693 at 1/100 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount™. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Counterstained with DAPI.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).