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Rabbit Recombinant Monoclonal CD21 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Mouse samples.

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Images

Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (AB324036), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (AB324036), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (AB324036), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Target data

Function

Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production.

Additional Targets

CR1

Alternative names

CD21, Complement receptor type 2, Cr2, Complement C3d receptor

Rabbit Recombinant Monoclonal CD21 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27417-513
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (ab324036), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (ab324036)

    CD21+CD35 Flow Cytometry staining using rabbit Anti-CD21+CD35 antibody

    Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / WEHI-231 (mouse B cell lymphoma B lymphocyte, Right) cells labelling CD35 + CD21 with ab324036 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Gated on viable cell.

    Negative control: EL4(PMID: 10453011)

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (ab324036), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (ab324036)

    CD21+CD35 Flow Cytometry staining of mouse splenocytes using rabbit Anti-CD21+CD35 antibody

    Flow cytometric analysis of mouse splenocytes labelling CD35 + CD21 with ab324036 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells are co-stained with CD3 conjugated to Alexa Fluor®488.

    Gated on viable cell.

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (ab324036), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD21+CD35 antibody [EPR27417-513] (ab324036)

    CD21+CD35 Flow Cytometry staining of mouse splenocytes using rabbit Anti-CD21+CD35 antibody

    Flow cytometric analysis of mouse splenocytes labelling CD35 + CD21 with ab324036 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells are co-stained with CD19 conjugated to PE/Cy7.

    Gated on viable cell.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com