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Rabbit Recombinant Monoclonal CD27 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), Flow Cyt and reacts with Transfected cell line - Mouse, Human, Mouse, Rat samples.

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Images

Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (AB324178), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (AB324178), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (AB324178), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (AB324178), expandable thumbnail
  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (AB324178), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)Flow CytICC/IF
Human
Expected
Tested
Not recommended
Mouse
Expected
Tested
Not recommended
Rat
Expected
Tested
Not recommended
Transfected cell line - Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Mouse
Dilution info
1/5000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Transfected cell line - Mouse
Dilution info
-
Notes

-

Target data

Function

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed:15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed:38386557).

Alternative names

CD27, Tnfrsf7, Cd27, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7

Rabbit Recombinant Monoclonal CD27 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), Flow Cyt and reacts with Transfected cell line - Mouse, Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27428-9
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.

Biological function summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Pathways

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

Associated diseases and disorders

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178)

    CD27 Flow Cytometry staining of Rat PBMC (Rat peripheral blood mononuclear cell) using rabbit Anti-CD27 antibody

    Flow cytometric analysis of Rat PBMC (Rat peripheral blood mononuclear cell) cells labelling CD27 with ab324178 at 1/5000 dilution (0.01ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells were co-stained with anti CD3 conjugated to Alexa Fluor®488.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178)

    CD27 Flow Cytometry staining of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) using rabbit Anti-CD27 antibody

    Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD27 with ab324178 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178)

    CD27 Flow Cytometry staining of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) using rabbit Anti-CD27 antibody

    Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD27 with ab324178 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells were co-stained with anti CD3 conjugated to Alexa Fluor®488.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178)

    CD27 Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-CD27 antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with ab324178 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells were co-stained with anti CD11b conjugated to Brilliant Violet 421.

    Gated on viable cells.

  • Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178)

    CD27 Flow Cytometry staining of Human PBMC (human peripheral blood mononuclear cell) using rabbit Anti-CD27 antibody

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with ab324178 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells were co-stained with anti CD3 conjugated to Alexa Fluor®488.

    Gated on viable cells.

  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD27 antibody [EPR27428-9] (ab324178)

    CD27 Flow Cytometry (Intracellular) staining using rabbit Anti-CD27 antibody

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector (Middle) / 293T cells transfected with an empty expression vector (Right) cells labelling CD27 with ab324178 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control.

    Cells were co-stained with Myc tag conjugated to Alexa Fluor®488.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com