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AB310016

Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

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Rabbit Recombinant Monoclonal CD3 zeta phospho Y142 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (AB310016)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (AB310016)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling CD3 zeta (phospho Y142) with ab310016 at 1/50 dilution (10.0 µg/ml). ab195887, Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used at 1/200 dilution (2.5 µg/ml) for Tubulin counter stanining (Green). DAPI was used as nuclear counter stain. Confocal image showing increased cytoplasmic staining in Jurkat cells treated with pervanadate (1 mM) for 30 min. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (AB310016)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (AB310016)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) treated with 1mM Pervanadate for 30min (Red) / Untreated Jurkat (Green) cells labelling CD3 zeta (phospho Y142) with ab310016 at 1/500 dilution (0.1 ug) /(Red and Green) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

  • Unconjugated

    Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

  • 660 APC

    APC Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

  • 578 PE

    PE Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

  • Carrier free

    Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP265(2)Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 1384049, PubMed : 1385158, PubMed : 2470098, PubMed : 7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed : 7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
See full target information CD247 phospho Y142
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB245765

Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free

primary-antibodies

cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765

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Alternative Version
Primary Antibodies

AB311910

Alexa Fluor® 555 Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y]

primary-antibodies

alexa-fluor-555-cd3-zeta-phospho-y142-antibody-ep2652y-ab311910

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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