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AB237452

Alexa Fluor® 647 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

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(1 Publication)

Rabbit Recombinant Monoclonal CD3 zeta phospho Y83 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237452)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237452)

ab237452 staining CD3 zeta in Jurkat cells +/- pervanadate (1mM, 30min). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237452 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237452)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] (AB237452)

Overlay histogram showing serum-starved Jurkat cells (Human epidermoid carcinoma epithelial cell) treated (red line) or untreated (green line) with 1 mM pervanadate for 4 hours. Cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol. The cells were then incubated in 1x PBS/10% normal serum to block non-specific protein-protein interactions followed by the antibody (ab237452, red and green lines) (1x106 in 100μl at 1/500 dilution (1 μg/ml)) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 17 mW Red laser (633 nm) and 660/20 bandpass filter.

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 519 FITC

    FITC Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • Unconjugated

    Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y]

  • Carrier free

    Anti-CD3 zeta (phospho Y83) antibody [EP776(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP776(2)Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in pervanadate (1mM, 30min) treated Jurkat fixed with 4% formaldehyde (10 min).</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 1384049, PubMed : 1385158, PubMed : 2470098, PubMed : 7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed : 7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
See full target information CD247 phospho Y83
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 50:6687-6701 PubMed35713529

2022

Genome-wide CRISPR/Cas9 transcriptional activation screen identifies a histone acetyltransferase inhibitor complex as a regulator of HIV-1 integration.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhang,Shaobo Wang,Wanyu Li,Edwin Yau,Hui Hui,Parmit Kumar Singh,Vasudevan Achuthan,Maile Ann Young Karris,Alan N Engelman,Tariq M Rana
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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