Alexa Fluor® 647 Anti-CD31 antibody [EPR17259]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR17259] (AB305210)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling CD31 with ab305210 at 1/100 (5.0 ug/ml). Positive staining on endothelial cells of human lung. The section was incubated with ab305210 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Negative control : PBS instead of the primary antibody.

• mIHC

Collaborator

Multiplex immunohistochemistry - Alexa Fluor® 647 Anti-CD31 antibody [EPR17259] (AB305210)

Immunofluorescence analysis of human rectum adenocarcinoma labelled with ab305210. Image was acquired using Cell DIVE Multiplexed Imaging Solution (Leica Microsystems).

Formalin fixed paraffin embedded (FFPE) sections were incubated with ab305210 at 1/100 dilution, for 1h at room temperature. Nuclear DNA was labelled with DAPI (Red). Deparaffinization, antigen retrieval, biomarker labeling and imaging was performed following Cell DIVE recommended standard protocol. Cell DIVE automatically processed the image to perform autofluorescence removal, distortion correction, blank glass subtraction, flat-field correction, and stitching.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR17259] (AB305210)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling CD31 with ab305210 at 1/100 (5.0 ug/ml). Positive staining on rat lung. The section was incubated with ab305210 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Negative control : PBS instead of the primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR17259] (AB305210)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD31 with ab305210 at 1/100 (5.0 ug/ml). Positive staining on endothelial cells of mouse lung. The section was incubated with ab305210 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Negative control : PBS instead of the primary antibody.