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AB218582

Alexa Fluor® 647 Anti-CD31 antibody [EPR3094]

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(7 Publications)

Anti-CD31 antibody [EPR3094] - Alexa Fluor® 647 conjugated (ab218582) is a rabbit recombinant monoclonal antibody detecting CD31 in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] (AB218582)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] (AB218582)

IHC image of CD31 staining in a section of formalin-fixed paraffin-embedded normal human kidney*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 8 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab218582 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3094

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>The ideal fixation time will depend on the size of the tissue block and the type of tissue, but fixation between 18–24h is suitable for most samples.</p><p>Positive Control: Hu tonsil tissue</p>" } } }
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You may be interested in:

AB204527

Mouse CD31 ELISA Kit (PECAM1)

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-CD31 antibody [EPR3094] (ab218582) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Related products
Antibody clone EPR3094 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD31 Alexa Fluor® 647 [EPR3094] (ab218582).

Other related products
We have a range of other formats of antibody clone [EPR3094] also available for your convenience: ab76533, Biotin - ab199734, Carrier free - ab207090, Alexa Fluor® 647 - ab218582, Alexa Fluor® 488 - ab275989, Alexa Fluor® 594 - ab277231, Alexa Fluor® 555 - ab279331, ab284419

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer (Dove Medical Press) 15:101-119 PubMed36761696

2023

An Magnetic-Targeting Nano-Diagnosis and Treatment Platform for TNBC.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Zhang,Shengxian Bao,Guanhua Qiu,Jingchen Liang,Qin Wang,Xiaoqi Zhu,Guchun Qin,Junjie Liu,Chang Zhao

Cell genomics 2: PubMed36419822

2022

Spatial intra-tumor heterogeneity is associated with survival of lung adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Jun Wu,Daniel Temko,Zoltan Maliga,Andre L Moreira,Emi Sei,Darlan Conterno Minussi,Jamie Dean,Charlotte Lee,Qiong Xu,Guillaume Hochart,Connor A Jacobson,Clarence Yapp,Denis Schapiro,Peter K Sorger,Erin H Seeley,Nicholas Navin,Robert J Downey,Franziska Michor

Cell reports. Medicine 3:100525 PubMed35243422

2022

An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Brett E Johnson,Allison L Creason,Jayne M Stommel,Jamie M Keck,Swapnil Parmar,Courtney B Betts,Aurora Blucher,Christopher Boniface,Elmar Bucher,Erik Burlingame,Todd Camp,Koei Chin,Jennifer Eng,Joseph Estabrook,Heidi S Feiler,Michael B Heskett,Zhi Hu,Annette Kolodzie,Ben L Kong,Marilyne Labrie,Jinho Lee,Patrick Leyshock,Souraya Mitri,Janice Patterson,Jessica L Riesterer,Shamilene Sivagnanam,Julia Somers,Damir Sudar,Guillaume Thibault,Benjamin R Weeder,Christina Zheng,Xiaolin Nan,Reid F Thompson,Laura M Heiser,Paul T Spellman,George Thomas,Emek Demir,Young Hwan Chang,Lisa M Coussens,Alexander R Guimaraes,Christopher Corless,Jeremy Goecks,Raymond Bergan,Zahi Mitri,Gordon B Mills,Joe W Gray

Nature communications 12:7292 PubMed34911933

2021

Epigenetic loss of heterogeneity from low to high grade localized prostate tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Sebnem Ece Eksi,Alex Chitsazan,Zeynep Sayar,George V Thomas,Andrew J Fields,Ryan P Kopp,Paul T Spellman,Andrew C Adey

Cell reports methods 1: PubMed34485971

2021

Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms.

Applications

Unspecified application

Species

Unspecified reactive species

Erik A Burlingame,Jennifer Eng,Guillaume Thibault,Koei Chin,Joe W Gray,Young Hwan Chang

Cell 183:1354-1366.e13 PubMed33065030

2020

Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Malika Aid,Kathleen Busman-Sahay,Samuel J Vidal,Zoltan Maliga,Stephen Bondoc,Carly Starke,Margaret Terry,Connor A Jacobson,Linda Wrijil,Sarah Ducat,Olga R Brook,Andrew D Miller,Maciel Porto,Kathryn L Pellegrini,Maria Pino,Timothy N Hoang,Abishek Chandrashekar,Shivani Patel,Kathryn Stephenson,Steven E Bosinger,Hanne Andersen,Mark G Lewis,Jonathan L Hecht,Peter K Sorger,Amanda J Martinot,Jacob D Estes,Dan H Barouch

bioRxiv : the preprint server for biology : PubMed32935109

2020

Age-dependent regulation of SARS-CoV-2 cell entry genes and cell death programs correlates with COVID-19 disease severity.

Applications

Unspecified application

Species

Unspecified reactive species

Zintis Inde,Clarence Yapp,Gaurav N Joshi,Johan Spetz,Cameron Fraser,Brian Deskin,Elisa Ghelfi,Chhinder Sodhi,David J Hackam,Lester Kobzik,Ben A Croker,Douglas Brownfield,Hongpeng Jia,Kristopher A Sarosiek
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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