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Mouse Monoclonal CD31 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

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Images

Immunocytochemistry - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912), expandable thumbnail

Publications

  • Bioengineering (Basel, Switzerland) 10:2023
    Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Alicia Y Lim,Yuki Kato,Courtney Sakolish,Alan Valdiviezo,Gang Han,Piyush Bajaj,Jason Stanko,Stephen S Ferguson,Remi Villenave,Philip Hewitt,Rhiannon N Hardwick,Ivan Rusyn
    PubMed 37892925
  • Nature communications 14:33952023
    Transparent tissue in solid state for solvent-free and antifade 3D imaging.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Fu-Ting Hsiao,Hung-Jen Chien,Ya-Hsien Chou,Shih-Jung Peng,Mei-Hsin Chung,Tzu-Hui Huang,Li-Wen Lo,Chia-Ning Shen,Hsiu-Pi Chang,Chih-Yuan Lee,Chien-Chia Chen,Yung-Ming Jeng,Yu-Wen Tien,Shiue-Cheng Tang
    PubMed 37296117

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow Cyt (Intra)
Human
Tested
Tested
Cynomolgus monkey
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/100

Notes

This product gave a positive signal in HUVEC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Use 0.3M glycine to quench autofluorescence caused by aldehydes.

Positive Control: HUVEC cells

Predicted
Predicted

Species

Cynomolgus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/5000.00000 - 1/12500.00000

Notes

-

Predicted
Predicted

Species

Cynomolgus monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Version

    8 products for Alternative Product

      Target data

      Function

      Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).Isoform Delta15Does not protect against apoptosis.

      Alternative names

      Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

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      Mouse Monoclonal CD31 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

      Alternative names

      Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

      Key facts

      Isotype

      IgG1

      Conjugation

      Alexa Fluor® 647

      Excitation/Emission

      Ex: 650nm, Em: 665nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      JC/70A

      Purification technique

      Affinity purification Immunogen

      Light chain type

      kappa

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      2 product images

      • Immunocytochemistry - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912), expandable thumbnail

        Immunocytochemistry - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912)

        ab215912 staining CD31 in HUVEC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215912 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

        This product also gave a positive signal under the same testing conditions in HUVEC cells fixed with 4% formaldehyde (10 min).

      • Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912), expandable thumbnail

        Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912)

        Overlay histogram showing HUVEC cells stained with ab215912 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215912, 1/5000 dilution) for 30 min at 22°C.

        Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

        This antibody gave a positive signal in HUVEC cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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