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AB215912

Alexa Fluor® 647 Anti-CD31 antibody [JC/70A]

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(1 Review)

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(14 Publications)

Anti-CD31 antibody [JC/70A] - Alexa Fluor® 647 conjugated (ab215912) is a mouse monoclonal antibody detecting CD31 in Flow Cytometry (Intra). Suitable for Human.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

2 Images
Immunocytochemistry - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912)
  • ICC

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912)

ab215912 staining CD31 in HUVEC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215912 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HUVEC cells fixed with 4% formaldehyde (10 min).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (AB215912)

Overlay histogram showing HUVEC cells stained with ab215912 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab215912, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in HUVEC cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

JC/70A

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p>This product gave a positive signal in HUVEC cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p><p>Use 0.3M glycine to quench autofluorescence caused by aldehydes.</p><p>Positive Control: HUVEC cells</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000 - 1/12500", "FlowCytIntra-species-notes": "<p></p>" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Product Specifications
Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC in human samples.

Quality and Validation
Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912) has been cited over 5 times in peer reviewed journals and is trusted by the scientific community.
Flourescent conjugated antibodies are ideal tools for multiplex IHC and flow cytometry experiments.
Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912) has 5 independent reviews from customers.
Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] (ab215912) specifically detects CD31 (UniProt ID: P16284; Molecular weight: 80kDa) and is sold in 100 µL selling sizes.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40627458

2025

Tumor microenvironments with an active type I IFN response are sensitive to inhibitors of heme degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Dominika Sosnowska,Tik Shing Cheung,Jit Sarkar,James W Opzoomer,Karen T Feehan,Joanne E Anstee,Chloé A Woodman,Mohamed Reda Keddar,Kalum Clayton,Samira Ali,William Macmorland,Dorothy D Yang,James Rosekilly,Cheryl E Gillett,Francesca D Ciccarelli,Richard Buus,James Spicer,Anita Grigoriadis,James N Arnold

Scientific reports 15:12414 PubMed40216867

2025

Liver quad culture chip as a model for radiation injury research.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Ueda,Satoshi Omiya,Jonathan Pinney,Michelle A Bylicky,Molykutty J Aryankalayil

Small methods 9:e2401588 PubMed39871784

2025

Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo-Epidermal Skin Substitutes.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic Rütsche,Monica Nanni,Phil Cheng,Nicolà Caflisch,Aizhan Tastanova,Céline Jenni,Mitchell P Levesque,Ueli Moehrlen,Agnes S Klar,Thomas Biedermann

Nature medicine 31:502-513 PubMed39775038

2025

Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Muñoz-Blat,Raúl Pérez-Moraga,Nerea Castillo-Marco,Teresa Cordero,Ana Ochando,Sheila Ortega-Sanchís,Marcos Parras-Moltó,Rogelio Monfort-Ortiz,Elena Satorres-Perez,Blanca Novillo,Alfredo Perales,Matthew Gormley,Sofia Granados-Aparici,Rosa Noguera,Beatriz Roson,Susan J Fisher,Carlos Simón,Tamara Garrido-Gómez

Transplantation direct 10:e1705 PubMed39399058

2024

Spatial Transcriptomic Signatures of Early Acute T Cell-mediated Rejection in Kidney Transplants.

Applications

Unspecified application

Species

Unspecified reactive species

Minji Kang,Haeyoon Kwon,Jeongin Song,Yunyoung Jang,Seung Hee Yang,Seung-Min Cha,Ji Hwan Moon,Yong Chul Kim,Hyun Je Kim

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:5590-5606 PubMed38958537

2024

Neurovascular coupling, functional connectivity, and cerebrovascular endothelial extracellular vesicles as biomarkers of mild cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron D Owens,Camila Bonin Pinto,Peter Mukli,Rafal Gulej,Faddi Saleh Velez,Sam Detwiler,Lauren Olay,Jordan R Hoffmeister,Zsofia Szarvas,Mihaly Muranyi,Anna Peterfi,Ana Clara da C Pinaffi-Langley,Cheryl Adams,Jason Sharps,Zalan Kaposzta,Calin I Prodan,Angelia C Kirkpatrick,Stefano Tarantini,Anna Csiszar,Zoltan Ungvari,Ann L Olson,Guangpu Li,Priya Balasubramanian,Veronica Galvan,Andrew Bauer,Zachary A Smith,Tarun W Dasari,Shawn Whitehead,Manoj R Medapti,Fanny M Elahi,Aikaterini Thanou,Andriy Yabluchanskiy

American journal of physiology. Renal physiology 327:F91-F102 PubMed38721662

2024

Validation of an organ mapping antibody panel for cyclical immunofluorescence microscopy on normal human kidneys.

Applications

Unspecified application

Species

Unspecified reactive species

Maya Brewer,Lukasz G Migas,Kelly A Clouthier,Jamie L Allen,David M Anderson,Ellie Pingry,Melissa Farrow,Ellen M Quardokus,Jeffrey M Spraggins,Raf Van de Plas,Mark P de Caestecker

Cell reports. Medicine 4:101307 PubMed38056464

2023

Bexmarilimab-induced macrophage activation leads to treatment benefit in solid tumors: The phase I/II first-in-human MATINS trial.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna H Rannikko,Loic Verlingue,Maria de Miguel,Annika Pasanen,Debbie Robbrecht,Tanja Skytta,Sanna Iivanainen,Shishir Shetty,Yuk Ting Ma,Donna M Graham,Sukeshi Patel Arora,Panu Jaakkola,Christina Yap,Yujuan Xiang,Jami Mandelin,Matti K Karvonen,Juho Jalkanen,Sinem Karaman,Jussi P Koivunen,Anna Minchom,Maija Hollmén,Petri Bono

Bioengineering (Basel, Switzerland) 10: PubMed37892925

2023

Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations.

Applications

Unspecified application

Species

Unspecified reactive species

Alicia Y Lim,Yuki Kato,Courtney Sakolish,Alan Valdiviezo,Gang Han,Piyush Bajaj,Jason Stanko,Stephen S Ferguson,Remi Villenave,Philip Hewitt,Rhiannon N Hardwick,Ivan Rusyn

Nature communications 14:3395 PubMed37296117

2023

Transparent tissue in solid state for solvent-free and antifade 3D imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Ting Hsiao,Hung-Jen Chien,Ya-Hsien Chou,Shih-Jung Peng,Mei-Hsin Chung,Tzu-Hui Huang,Li-Wen Lo,Chia-Ning Shen,Hsiu-Pi Chang,Chih-Yuan Lee,Chien-Chia Chen,Yung-Ming Jeng,Yu-Wen Tien,Shiue-Cheng Tang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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