Alexa Fluor® 647 Anti-CD34 antibody [EPR27431-71]

5 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD34 antibody [EPR27431-71] (ab323893)

  CD34 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse liver using rabbit Anti-CD34 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CD34 with ab323893 at 1/100 (5.0 ug/ml) dilution.

  Negative control: no staining on mouse liver.
  
  The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  
  The immunostaining was performed on a Leica Biosystems BOND RX instrument.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Counterstained with DAPI.

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD34 antibody [EPR27431-71] (ab323893)

  CD34 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Rat pancreas using rabbit Anti-CD34 antibody

  Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling CD34 with ab323893 at 1/100 (5.0 ug/ml) dilution.

  Positive staining on endothelial cells of rat pancreas.
  
  The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  
  The immunostaining was performed on a Leica Biosystems BOND RX instrument.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Counterstained with DAPI.

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD34 antibody [EPR27431-71] (ab323893)

  CD34 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-CD34 antibody

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling CD34 with ab323893 at 1/100 (5.0 ug/ml) dilution.

  Positive staining on basement membrane of mouse testis.
  
  The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  
  The immunostaining was performed on a Leica Biosystems BOND RX instrument.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Counterstained with DAPI.

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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  Flow Cytometry - Alexa Fluor® 647 Anti-CD34 antibody [EPR27431-71] (ab323893)

  CD34 Flow Cytometry staining of Mouse bone marrow using rabbit Anti-CD34 antibody

  Flow cytometric analysis of Mouse bone marrow cells labelling CD34 with ab323893 at 1/500 dilution (0.1ug) / Right compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Gated on viable cells.
  
  Cells are co-stained with CD117 conjugated to APC-eFluor 780. (PMID: 23295606)

• 

  Flow Cytometry - Alexa Fluor® 647 Anti-CD34 antibody [EPR27431-71] (ab323893)

  CD34 Flow Cytometry staining using rabbit Anti-CD34 antibody

  Flow cytometric analysis of Neuro-2a (mouse neuroblastoma neuroblast, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling CD34 with ab323893 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (Alexa Fluor® 647 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199093) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Negative control: Neuro-2a.
  
  Gated on viable cells.