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AB198937

Alexa Fluor® 647 Anti-CD3D antibody [EP4426]

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(4 Publications)

Rabbit Recombinant Monoclonal CD3d antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CD3D with ab198937 at 1/100 (5.0 µg/ml) dilution.

Positive staining on human tonsil.
The primary antibody was incubated overnight at 4µC (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte, Left) and Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Right) cells labelling CD3D with ab198937 at 1/5000 dilution (0.01ug) / Red compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : THP-1.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CD3D with ab198937 at 1/100 (5.0 µg/ml) dilution.

Negative control : no staining on human pancreas.

The primary antibody was incubated overnight at 4µC (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD3D antibody [EP4426] (AB198937)

ab198937 staining CD3D in Jurkat cells. The cells were fixed with 80% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198937 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD3D antibody [EP4426]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD3D antibody [EP4426]

  • 578 PE

    PE Anti-CD3D antibody [EP4426]

  • 660 APC

    APC Anti-CD3D antibody [EP4426]

  • HRP

    HRP Anti-CD3D antibody [EP4426]

  • Unconjugated

    Anti-CD3D antibody [EP4426]

  • Carrier free

    Anti-CD3D antibody [EP4426] - Low endotoxin, Azide free

  • Carrier free

    Anti-CD3D antibody [EP4426] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP4426

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/5000", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in Jurkat cells fixed with 100% methanol (5 min)</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 23:100750 PubMed38513891

2024

An Automated and Fast Sample Preparation Workflow for Laser Microdissection Guided Ultrasensitive Proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Anuar Makhmut,Di Qin,David Hartlmayr,Anjali Seth,Fabian Coscia

Cell systems 14:1002-1014.e5 PubMed37909047

2023

A framework for ultra-low-input spatial tissue proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Anuar Makhmut,Di Qin,Sonja Fritzsche,Jose Nimo,Janett König,Fabian Coscia

Cell 183:1354-1366.e13 PubMed33065030

2020

Vascular Disease and Thrombosis in SARS-CoV-2-Infected Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Malika Aid,Kathleen Busman-Sahay,Samuel J Vidal,Zoltan Maliga,Stephen Bondoc,Carly Starke,Margaret Terry,Connor A Jacobson,Linda Wrijil,Sarah Ducat,Olga R Brook,Andrew D Miller,Maciel Porto,Kathryn L Pellegrini,Maria Pino,Timothy N Hoang,Abishek Chandrashekar,Shivani Patel,Kathryn Stephenson,Steven E Bosinger,Hanne Andersen,Mark G Lewis,Jonathan L Hecht,Peter K Sorger,Amanda J Martinot,Jacob D Estes,Dan H Barouch

Molecular immunology 111:209-219 PubMed31096062

2019

The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Noureddine Ben Khalaf,Wedad Al-Mashoor,Azhar Saeed,Dalal Al-Mehatab,Safa Taha,Moiz Bakhiet,M Dahmani Fathallah
View all publications
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Product promise

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