AB196147

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

Name: Alexa Fluor® 647 conjugated Anti-CD4 antibody [EPR6855] (ab196147) | Abcam
Brand: Abcam Limited
SKU: AB196147
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(16 Publications)

Anti-CD4 antibody [EPR6855] - Alexa Fluor® 647 conjugated (ab196147) is a rabbit recombinant monoclonal antibody detecting CD4 in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

Immunofluorescence staining of CD4 in a section of formalin-fixed paraffin-embedded human normal tonsil*.

The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab196147 at 1/100 dilution (shown in red) and counterstained using ab17147 Ms anti-CD8 at 1/100 dilution. The section was then incubated with ab150118 ((Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preabsorbed) 1/1000, shown in green) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

HRP

HRP Anti-CD4 antibody [EPR6855]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD4 antibody [EPR6855]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD4 antibody [EPR6855]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]
Unconjugated

Anti-CD4 antibody [EPR6855]
Carrier free

Anti-CD4 antibody [EPR6855] – Mouse IgG1 (Chimeric) – BSA and Azide Free
Carrier free

Anti-CD4 antibody [EPR6855] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6855

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (ab196147) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Related products
Antibody clone EPR6855 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD4 Alexa Fluor® 647 [EPR6855] (ab196147).

Other related products
We have a range of other formats of antibody clone [EPR6855] also available for your convenience: ab133616, Carrier free - ab181724, HRP - ab195842, Alexa Fluor® 647 - ab196147, Alexa Fluor® 488 - ab196372, Alexa Fluor® 594 - ab277931, Alexa Fluor® 555 - ab280849, ab284581, Carrier free - ab307492, ab317787, Carrier free - ab317797

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).

See full target information CD4

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:103946 PubMed40652508

2025

Protocol for studying the immune microenvironment of human hepatocellular carcinoma by Cell DIVE multiplex immunofluorescence imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Margaux Delaporte,Maelle Guillout,Pascale Bellaud,Anthony Sébillot,Bruno Turlin,Thierry Pécot,Michel Samson,Céline Raguénès-Nicol

iScience 27:110668 PubMed39246442

2024

Multiplex imaging of localized prostate tumors reveals altered spatial organization of AR-positive cells in the microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Çiğdem Ak,Zeynep Sayar,Guillaume Thibault,Erik A Burlingame,M J Kuykendall,Jennifer Eng,Alex Chitsazan,Koei Chin,Andrew C Adey,Christopher Boniface,Paul T Spellman,George V Thomas,Ryan P Kopp,Emek Demir,Young Hwan Chang,Vasilis Stavrinides,Sebnem Ece Eksi

Inflammation 48:975-986 PubMed39174864

2024

Optimal Timing of PD-1/PD-L1 Blockade Protects Organ Function During Sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiang,Yuanyang Wang,Langtian Shi,Jinchao Qiu,Lebin Gan,Zhe Xu,Huacai Zhang,Jin Deng,Zhen Wang,Fang Xu,Ling Zeng

Advanced healthcare materials 14:e2401192 PubMed38837879

2024

T Cells Spatially Regulate B Cell Receptor Signaling in Lymphomas through H3K9me3 Modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy S Britto,Deepali Balasubramani,Sona Desai,Phunterion Phillips,Neev Trehan,Ethel Cesarman,Jean L Koff,Ankur Singh

The Journal of experimental medicine 221: PubMed38557723

2024

Helper T cell immunity in humans with inherited CD4 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Guérin,Marcela Moncada-Vélez,Katherine Jackson,Masato Ogishi,Jérémie Rosain,Mathieu Mancini,David Langlais,Andrea Nunez,Samantha Webster,Jesse Goyette,Taushif Khan,Nico Marr,Danielle T Avery,Geetha Rao,Tim Waterboer,Birgitta Michels,Esmeralda Neves,Cátia Iracema Morais,Jonathan London,Stéphanie Mestrallet,Pierre Quartier Dit Maire,Bénédicte Neven,Franck Rapaport,Yoann Seeleuthner,Atar Lev,Amos J Simon,Jorge Montoya,Ortal Barel,Julio Gómez-Rodríguez,Julio C Orrego,Anne-Sophie L'Honneur,Camille Soudée,Jessica Rojas,Alejandra C Velez,Irini Sereti,Benjamin Terrier,Nancy Marin,Luis F García,Laurent Abel,Stéphanie Boisson-Dupuis,Joel Reis,Antonio Marinho,Andrea Lisco,Emilia Faria,Christopher C Goodnow,Julia Vasconcelos,Vivien Béziat,Cindy S Ma,Raz Somech,Jean-Laurent Casanova,Jacinta Bustamante,Jose Luis Franco,Stuart G Tangye

Nature materials 22:511-523 PubMed36928381

2023

Combinatorial treatment rescues tumour-microenvironment-mediated attenuation of MALT1 inhibitors in B-cell lymphomas.

Applications

Unspecified application

Species

Unspecified reactive species

Shivem B Shah,Christopher R Carlson,Kristine Lai,Zhe Zhong,Grazia Marsico,Katherine M Lee,Nicole E Félix Vélez,Elisabeth B Abeles,Mayar Allam,Thomas Hu,Lauren D Walter,Karen E Martin,Khanjan Gandhi,Scott D Butler,Rishi Puri,Angela L McCleary-Wheeler,Wayne Tam,Olivier Elemento,Katsuyoshi Takata,Christian Steidl,David W Scott,Lorena Fontan,Hideki Ueno,Benjamin D Cosgrove,Giorgio Inghirami,Andrés J García,Ahmet F Coskun,Jean L Koff,Ari Melnick,Ankur Singh

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 36:100034 PubMed36788070

2023

Highly Multiplexed Spatially Resolved Proteomic and Transcriptional Profiling of the Glioblastoma Microenvironment Using Archived Formalin-Fixed Paraffin-Embedded Specimens.

Applications

Unspecified application

Species

Unspecified reactive species

Youngmi Kim,Patrick Danaher,Patrick J Cimino,Kyle Hurth,Sarah Warren,John Glod,Joseph M Beechem,Gabriel Zada,Troy A McEachron

Immunity 55:1799-1812.e4 PubMed36070769

2022

ABCC1 transporter exports the immunostimulatory cyclic dinucleotide cGAMP.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna H Maltbaek,Stephanie Cambier,Jessica M Snyder,Daniel B Stetson

Frontiers in immunology 13:850616 PubMed35479072

2022

Contact-Dependent Granzyme B-Mediated Cytotoxicity of Th17-Polarized Cells Toward Human Oligodendrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Hélène Jamann,Qiao-Ling Cui,Haritha L Desu,Florian Pernin,Olivier Tastet,Alexandre Halaweh,Negar Farzam-Kia,Victoria Hannah Mamane,Oumarou Ouédraogo,Aurélie Cleret-Buhot,Audrey Daigneault,Renaud Balthazard,Wendy Klement,Florent Lemaître,Nathalie Arbour,Jack Antel,Jo Anne Stratton,Catherine Larochelle

Cell reports. Medicine 3:100525 PubMed35243422

2022

An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Brett E Johnson,Allison L Creason,Jayne M Stommel,Jamie M Keck,Swapnil Parmar,Courtney B Betts,Aurora Blucher,Christopher Boniface,Elmar Bucher,Erik Burlingame,Todd Camp,Koei Chin,Jennifer Eng,Joseph Estabrook,Heidi S Feiler,Michael B Heskett,Zhi Hu,Annette Kolodzie,Ben L Kong,Marilyne Labrie,Jinho Lee,Patrick Leyshock,Souraya Mitri,Janice Patterson,Jessica L Riesterer,Shamilene Sivagnanam,Julia Somers,Damir Sudar,Guillaume Thibault,Benjamin R Weeder,Christina Zheng,Xiaolin Nan,Reid F Thompson,Laura M Heiser,Paul T Spellman,George Thomas,Emek Demir,Young Hwan Chang,Lisa M Coussens,Alexander R Guimaraes,Christopher Corless,Jeremy Goecks,Raymond Bergan,Zahi Mitri,Gordon B Mills,Joe W Gray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-CD4 antibody [EPR6855]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (AB196147)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (16)

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