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AB302830

Alexa Fluor® 647 Anti-CD40 antibody [EPR20735]

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Rabbit Recombinant Monoclonal CD40 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD40 antibody [EPR20735] (AB302830)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD40 antibody [EPR20735] (AB302830)

Immunocytochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells staining CD40 using ab302830 at a 1/50 dilution (10.0 μg/ml) (Red). Counterstained with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) at a 1/200 dilution (2.5 μg/ml) (Green). Nuclear counterstain is DAPI (Blue). Confocal image showing membranous and weakly cytoplasmic staining in Raji cells. Negative control : Jurkat (PMID : 10498643).

Flow Cytometry - Alexa Fluor® 647 Anti-CD40 antibody [EPR20735] (AB302830)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 647 Anti-CD40 antibody [EPR20735] (AB302830)

Flow Cytometry analysis of Jurkat (human T cell leukemia T lymphocyte) cells (Left panel) or Raji (human Burkitt's lymphoma B lymphocyte (Right panel) cells stained for CD40 using ab302830 at a 1/500 dilution (0.1 μg) (Red) compared to Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 647) (ab199093) (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Gated on viable cells. Negative control : Jurkat (PMID : 10498643).

  • Unconjugated

    Anti-CD40 antibody [EPR20735]

  • Carrier free

    Anti-CD40 antibody [EPR20735] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD40 antibody [EPR20735]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD40 antibody [EPR20735]

  • 660 APC

    APC Anti-CD40 antibody [EPR20735]

  • 578 PE

    PE Anti-CD40 antibody [EPR20735]

  • 519 FITC

    FITC Anti-CD40 antibody [EPR20735]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20735

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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AB99990

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF5/CD40LG (PubMed : 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
See full target information CD40
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Version
Primary Antibodies

AB228818

Anti-CD40 antibody [EPR20735] - BSA and Azide free

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Alternative Version
Primary Antibodies

AB302971

Alexa Fluor® 488 Anti-CD40 antibody [EPR20735]

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Alternative Version
Primary Antibodies

AB314949

FITC Anti-CD40 antibody [EPR20735]

primary-antibodies

fitc-cd40-antibody-epr20735-ab314949

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com