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AB310264

Alexa Fluor® 647 Anti-CD53 antibody [EPR4342(2)]

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Rabbit Recombinant Monoclonal CD53 antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

CD53, MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25

  • 578 PE

    PE Anti-CD53 antibody [EPR4342(2)]

  • 660 APC

    APC Anti-CD53 antibody [EPR4342(2)]

  • HRP

    HRP Anti-CD53 antibody [EPR4342(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD53 antibody [EPR4342(2)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD53 antibody [EPR4342(2)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD53 antibody [EPR4342(2)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD53 antibody [EPR4342(2)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CD53 antibody [EPR4342(2)]

  • Unconjugated

    Anti-CD53 antibody [EPR4342(2)]

  • Carrier free

    Anti-CD53 antibody [EPR4342(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4342(2)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD53 also known as Tetraspanin-25 is a transmembrane glycoprotein with a molecular mass of approximately 35-40 kDa. It belongs to the tetraspanin family characterized by four transmembrane domains. CD53 primarily resides on the surface of leukocytes including B cells T cells natural killer cells and macrophages. This protein plays an important role in signal transduction processes by organizing proteins on the cell membrane and forming tetraspanin-enriched microdomains.
Biological function summary

CD53 plays a significant role in regulating immune responses cell development and migration. It does not work alone but as part of larger protein complexes that influence cell communication and inter-organizational responses. CD53 participates in organizing membrane proteins into functional complexes which impacts cellular processes such as adhesion signaling and cellular activation. Its interaction with other proteins is critical to maintain normal immune system functionality and responding appropriately to infections.

Pathways

CD53 is involved in the regulation of immune signaling and cellular communication networks. It is a component of the tetraspanin web which influences the integrin and immunoreceptor pathways two important signaling pathways in cellular communication. Proteins such as CD9 and CD81 also members of the tetraspanin family frequently interact with CD53 suggesting cooperative roles in modulating cellular adhesion and trafficking processes critical for immune response.

CD53 shows a connection to immune system dysfunctions including autoimmune conditions and certain types of cancer. Its altered expression can contribute to abnormal immune responses seen in diseases like systemic lupus erythematosus (SLE) and lymphoma. In these disorders CD53's interaction with CD19 and other B-cell-associated proteins plays a part in abnormal cell signaling leading to issues in cell proliferation or miscommunication within the immune system.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 28487417). Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed : 32974937, PubMed : 35767951). Plays a role in the activation of monocytes and B-cells (PubMed : 8335905). Acts as an essential regulator of B-cell development by promoting interleukin-7 receptor/IL7R signaling (By similarity). Promotes also in B-cells the BCR signaling by recruiting PKC to the plasma membrane in order to phosphorylate its substrates (PubMed : 28487417). Plays an essential role in B- and T-cells homing to lymph nodes by stabilizing L-selectin/SELL cell surface expression (By similarity). Mediates also metabolic and inflammatory functions in hepatocytes and adipose tissue by promoting TNF-alpha and LPS signaling independent of the immune compartment (By similarity).
See full target information CD53
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Product promise

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For full details, please see our Terms & Conditions

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