Mouse Monoclonal CD59 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD59 protein.
Images
![Flow Cytometry - Alexa Fluor® 647 Anti-CD59 antibody [MEM-43] (AB187769), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cd59-antibody-mem-43-ab187769--flow-cytometry-img92482.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Alexa Fluor® 647 Anti-CD59 antibody [MEM-43] (AB187769)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13987
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 4 µL for 106 Cells | Notes (or 100 μl of whole blood). ab176104 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Associated Products
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3 products for Alternative Product
Target data
Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Alternative names
CD59, MIC11, MIN1, MIN2, MIN3, MSK21, CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, MAC-inhibitory protein, MEM43 antigen, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin, HRF-20, HRF20, MAC-IP, MACIF, MIRL
Recommended products
Mouse Monoclonal CD59 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD59 protein.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 99% PBS, 0.2% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD59 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P13987
- Clone number
- MEM-43
- Purification technique
- Size-exclusion chromatography
- Specificity
ab187769 reacts with a well defined epitope (W40, R-53) on CD59.
- Concentration
- Purification notes
Purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD59 also known as protectin is a protein with a molecular mass of approximately 18-25 kDa. It is widely expressed on the surface of many human cells including red blood cells and various types of leukocytes. CD59 serves as an inhibitor of the complement membrane attack complex (MAC) preventing cell lysis caused by terminal complement proteins. Through this mechanism it regulates complement activation and maintains cell integrity by halting the formation of MAC.
- Biological function summary
One important feature of CD59 is its role as a glycosylphosphatidylinositol (GPI)-anchored protein involved in safeguarding cells from complement-mediated damage. It does not exist within a larger complex but functions at the cell surface to inhibit the assembly of complement components C5b-9 which form the MAC. This ability to inhibit MAC is essential in maintaining self-tissue from unintended damage during immune responses.
- Pathways
CD59 participates in the regulation of the complement cascade specifically within the terminal pathway. The complement system serves as a bridge between innate and adaptive immunity contributing to processes like opsonization and cell lysis. CD59's inhibitory action directly impacts pathways that utilize terminal components such as C5b. Effective CD59 function prevents excessive complement activation ensuring that an immune response does not damage host tissues. It links closely with other complement regulatory proteins like CD55 which also mitigate complement cascade activation.
- Associated diseases and disorders
Deficiencies or malfunctions in CD59 have notable implications. Paroxysmal nocturnal hemoglobinuria (PNH) is a disorder where lack of CD59 expression on blood cells leads to their increased destruction due to unregulated complement activity. Furthermore CD59's dysfunction or inadequacy also associates with atypical hemolytic uremic syndrome (aHUS) which involves mutant complement regulatory proteins causing overactivation of the complement system. These associations highlight the importance of CD59 in both maintaining cellular health and preventing pathophysiological conditions related to complement overactivity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Alexa Fluor® 647 Anti-CD59 antibody [MEM-43] (ab187769), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cd59-antibody-mem-43-ab187769--flow-cytometry-img92482.jpg "Flow Cytometry - Alexa Fluor® 647 Anti-CD59 antibody [MEM-43] (ab187769)")
Flow Cytometry - Alexa Fluor® 647 Anti-CD59 antibody [MEM-43] (ab187769)
CD59 Flow Cytometry staining using mouse Anti-CD59 antibody
Surface staining of HL-60 (positive) and SP2 (negative) cells with ab187769 1/25 dilution
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