AB192343

Alexa Fluor® 647 Anti-CD64 antibody [10.1]

Name: Anti-CD64 Alexa Fluor® 647 antibody [10.1] (ab192343) IgG1 | Abcam
Brand: Abcam Limited
SKU: AB192343
Price: 145 USD
Availability: InStock

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(2 Publications)

Mouse Monoclonal CD64 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt and reacts with Human, Primates samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing FCGR1A protein.

View Alternative Names

CD64, FCG1, FCGR1, IGFR1, FCGR1A, High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc-gamma RI, Fc-gamma RIA, FcRI, FcgammaRIa

1 Images

Flow Cytometry - Alexa Fluor® 647 Anti-CD64 antibody [10.1] (AB192343)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 647 Anti-CD64 antibody [10.1] (AB192343)

Flow Cytometry analysis of human peripheral blood cells labeling CD64 with Anti-CD64 [10.1] antibody, prediluted (Alexa Fluor® 647) (ab192343).

Unconjugated

Anti-CD64 antibody [10.1]
Unconjugated

Anti-CD64 antibody [10.1] - Low endotoxin, Azide free
667 PE/Cy5®

PE/Cy5® Anti-CD64 antibody [10.1]
778 PE/Cy7®

PE/Cy7® Anti-CD64 antibody [10.1]
519 FITC

FITC Anti-CD64 antibody [10.1]
578 PE

PE Anti-CD64 antibody [10.1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10.1

Isotype

IgG1

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Primates

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing FCGR1A protein. The exact immunogen used to generate this antibody is proprietary information.

P12314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>4μl/100 μl of whole bloodab176103 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>4μl/100 μl of whole bloodab176103 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Size-exclusion chromatography

Purification notes

Purified antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD64 also known as Fc gamma receptor 1a (FCGR1A) is a high-affinity receptor for the Fc region of immunoglobulin G (IgG). This receptor weighs approximately 72 kDa and is expressed on the surface of immune cells such as macrophages monocytes and activated neutrophils. CD64 protein plays an important role in immune responses by binding to antibodies and mediating processes such as phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). Researchers often use anti-CD64 antibodies and CD64 markers to study its expression in various cellular contexts especially in flow cytometry.

Biological function summary

CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.

Pathways

CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.

CD64 expression correlates strongly with autoimmune disorders and inflammatory conditions. The receptor's role in orchestrating immune cell activation makes it relevant in diseases such as rheumatoid arthritis and sepsis. In rheumatoid arthritis CD64 interacts with immune complexes intensifying inflammation through enhancement of phagocytic activity. In sepsis increased CD64 marker expression on neutrophils can serve as a diagnostic indicator. Related proteins like CD16 and CD32 also participate in these disorders by modulating immune complex handling and cellular activation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Mediates IgG effector functions on monocytes triggering antibody-dependent cellular cytotoxicity (ADCC) of virus-infected cells.

See full target information FCGR1A

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:258 PubMed38755644

2024

A novel conductive microtubule hydrogel for electrical stimulation of chronic wounds based on biological electrical wires.

Applications

Unspecified application

Species

Unspecified reactive species

Weijing Fan,Xiao Yang,Xiaoming Hu,Renyan Huang,Hongshuo Shi,Guobin Liu

Journal of biomedical science 23:90 PubMed27931227

2016

Epigenetics of amphetamine-induced sensitization: HDAC5 expression and microRNA in neural remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Philip K Liu,Christina H Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-CD64 antibody [10.1]

Flow Cytometry - Alexa Fluor® 647 Anti-CD64 antibody [10.1] (AB192343)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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