AB201845

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]

Name: Anti-CD68 Alexa Fluor® 647 antibody [FA-11] (ab201845) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB201845
Price: 530 USD
Availability: InStock

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(10 Publications)

Rat Monoclonal CD68 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Mouse samples. Cited in 10 publications.

View Alternative Names

CD68, Macrosialin, Cd68

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (AB201845)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (AB201845)

ab201845 staining CD68 in RAW264.7 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201845 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

660 APC

APC Anti-CD68 antibody [FA-11]
675 PerCP

PerCP Anti-CD68 antibody [FA-11]
HRP

HRP Anti-CD68 antibody [FA-11]
578 PE

PE Anti-CD68 antibody [FA-11]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-CD68 antibody [FA-11]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD68 antibody [FA-11]
Unconjugated

Anti-CD68 antibody [FA-11]
Carrier free

Anti-CD68 antibody [FA-11] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

FA-11

Isotype

IgG2a

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in RAW264.7 cells fixed with 100% methanol (5 min)</p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD68 is a glycoprotein that functions as a scavenger receptor. It plays an important role in the regulation of cellular debris and apoptotic cell clearance. Researchers often refer to it as KP1 or macrosialin in the literature. CD68 with a molecular weight of approximately 110 kDa is highly expressed in macrophages and monocytes. The mucin-like structure of CD68 allows it to be heavily glycosylated which aids in its function. Researchers detect CD68 in tissues through methods like immunohistochemistry (IHC) immunofluorescence and CD68 staining and it is widely used as a macrophage marker in research.

Biological function summary

CD68 facilitates functions associated with the innate immune response. It serves as an important part of macrophages which contribute to immune surveillance and tissue homeostasis. Though it primarily operates on its own in some contexts CD68 might assist in forming complexes with other receptors to modulate phagocytic activity. This target is significant in atherosclerotic plaque stability as macrophages engulf lipids and cell debris through processes facilitated by CD68.

Pathways

CD68 is involved in pathways connected to inflammation and phagocytosis. The CD68 protein works closely with other scavenger receptors like CD36 to mediate uptake of oxidized low-density lipoproteins (oxLDL) in the lipid metabolism pathway. Additionally CD68 engagement can influence the toll-like receptor (TLR) signaling pathways thereby linking innate immunity and inflammatory responses critical for host defense and disease progression.

CD68 is associated with atherosclerosis and multiple sclerosis. In atherosclerosis its role becomes evident as it accumulates in macrophages within the plaques making it a marker of disease severity. In multiple sclerosis CD68 expression in macrophages and microglia indicates active demyelination and inflammation. It also relates to proteins such as CD36 in atherosclerosis where both mediate the uptake of modified LDL contributing to foam cell formation in plaques.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

See full target information Cd68

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:113815 PubMed38428421

2024

Deficiency of lncRNA MERRICAL abrogates macrophage chemotaxis and diabetes-associated atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingshu Chen,Anurag Jamaiyar,Winona Wu,Yi Hu,Rulin Zhuang,Grasiele Sausen,Henry S Cheng,Camila de Oliveira Vaz,Daniel Pérez-Cremades,Aspasia Tzani,Michael G McCoy,Carmel Assa,Samuel Eley,Vinay Randhawa,Kwangwoon Lee,Jorge Plutzky,Naomi M Hamburg,Marc S Sabatine,Mark W Feinberg

Proceedings of the National Academy of Sciences of the United States of America 120:e2221249120 PubMed37410813

2023

A cellular and molecular spatial atlas of dystrophic muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Stec,Qi Su,Christina Adler,Lance Zhang,David R Golann,Naveen P Khan,Lampros Panagis,S Armando Villalta,Min Ni,Yi Wei,Johnathon R Walls,Andrew J Murphy,George D Yancopoulos,Gurinder S Atwal,Sandra Kleiner,Gabor Halasz,Mark W Sleeman

Disease markers 2022:4988539 PubMed36092961

2022

PIAS1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice through a Mechanism Involving NFATc1 SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Luo,Jiequn Li,Ting Li,Zhongqiang Zhang,Guangshun Chen,Qiang Li,Haizhi Qi,Zhongzhou Si

Acta pharmacologica Sinica 43:2817-2827 PubMed35501362

2022

Novel Caspase-1 inhibitor CZL80 improves neurological function in mice after progressive ischemic stroke within a long therapeutic time-window.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Pan,Wei-Dong Tang,Ke Wang,Qi-Feng Fang,Meng-Ru Liu,Zhan-Xun Wu,Yi Wang,Sun-Liang Cui,Gang Hu,Ting-Jun Hou,Wei-Wei Hu,Zhong Chen,Xiang-Nan Zhang

Cell reports 33:108346 PubMed33147450

2020

Microglial Displacement of GABAergic Synapses Is a Protective Event during Complex Febrile Seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Yushan Wan,Bo Feng,Yi You,Jie Yu,Cenglin Xu,Haibin Dai,Bruce D Trapp,Peng Shi,Zhong Chen,Weiwei Hu

Cell death & disease 11:459 PubMed32541671

2020

Brd4 inhibition ameliorates Pyocyanin-mediated macrophage dysfunction via transcriptional repression of reactive oxygen and nitrogen free radical pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Feimei Zhu,Feng Xiong,Jinchen He,Keyun Liu,Yuanyuan You,Qian Xu,Junming Miao,Yu Du,Lijuan Zhang,Hongyu Ren,Xiaoying Wang,Junli Chen,Jingyu Li,Shanze Chen,Xiaokang Liu,Ning Huang,Yi Wang

ACS infectious diseases 5:1996-2002 PubMed31345032

2019

Radiosynthesis and PET Bioimaging of Br-Bedaquiline in a Murine Model of Tuberculosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alvaro A Ordonez,Laurence S Carroll,Sudhanshu Abhishek,Filipa Mota,Camilo A Ruiz-Bedoya,Mariah H Klunk,Alok K Singh,Joel S Freundlich,Ronnie C Mease,Sanjay K Jain

PloS one 14:e0212880 PubMed30811469

2019

Deletion of suppressor of cytokine signaling 3 (SOCS3) in muscle stem cells does not alter muscle regeneration in mice after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kristy Swiderski,Marissa K Caldow,Timur Naim,Jennifer Trieu,Annabel Chee,René Koopman,Gordon S Lynch

Journal of neuroinflammation 15:155 PubMed29788960

2018

Ecto-5'-nucleotidase (CD73) attenuates inflammation after spinal cord injury by promoting macrophages/microglia M2 polarization in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Xu,Wei Zhu,Minghao Shao,Fan Zhang,Ji Guo,Haocheng Xu,Jianyuan Jiang,Xiaosheng Ma,Xinlei Xia,Xiuling Zhi,Ping Zhou,Feizhou Lu

Journal of the American Heart Association 7: PubMed29437605

2018

Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Guanmei Wen,Weiwei An,Jiangyong Chen,Eithne M Maguire,Qishan Chen,Feng Yang,Stuart W A Pearce,Maria Kyriakides,Li Zhang,Shu Ye,Sussan Nourshargh,Qingzhong Xiao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-CD68 antibody [FA-11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD68 antibody [FA-11] (AB201845)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise