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AB314339

Alexa Fluor® 647 Anti-CD98 antibody [EPR27110-42]

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Knockout Tested Rabbit Recombinant Monoclonal CD98 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

CD98, MDU1, SLC3A2, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain, 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, Solute carrier family 3 member 2, 4F2hc

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD98 antibody [EPR27110-42] (AB314339)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD98 antibody [EPR27110-42] (AB314339)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CD98 with ab314339 at 1/50 (10.0 ug/ml) dilution(Red). Confocal image showing membrane staining in HeLa cell line. Low expression : SH-SY5YImage was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Green). The Nuclear counterstain was DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD98 antibody [EPR27110-42] (AB314339)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD98 antibody [EPR27110-42] (AB314339)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SLC3A2 (CD98) KO HeLa (SLC3A2 (CD98) knockout human cervix adenocarcinoma epithelial cell) (ab265708) cells labelling CD98 with ab314339 at 1/50 (10.0 ug/ml) dilution(Red). Confocal image showing membrane staining in parental HeLa cell line, while no staining in SLC3A2 knockout HeLa cells (ab265708). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Green). The Nuclear counterstain was DAPI (Blue).

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD98 antibody [EPR27110-42]

  • 660 APC

    APC Anti-CD98 antibody [EPR27110-42]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD98 antibody [EPR27110-42]

  • 578 PE

    PE Anti-CD98 antibody [EPR27110-42]

  • Unconjugated

    Anti-CD98 antibody [EPR27110-42]

  • Carrier free

    Anti-CD98 antibody [EPR27110-42] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27110-42

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD98 also known as CD98hc CD98 protein or LAT1/CD98 is a type II membrane protein often found in association with the heavy chains of amino acid transporters like LAT1. It has a molecular mass of approximately 85-100 kDa. CD98 is widely expressed in numerous tissues including the liver kidney and various tumors. It plays an important role in cellular processes by facilitating the transport of amino acids across plasma membranes which is essential for maintaining cellular homeostasis and proliferation.
Biological function summary

CD98 forms a complex with light chains of the heterodimeric amino acid transporters such as LAT1 and LAT2. This interaction enhances the uptake of neutral amino acids into cells. CD98 also interacts with integrins influencing cell adhesion and migration which are critical for cellular signaling and response. Through these interactions CD98 impacts not only cell survival and growth but also immune responses and angiogenesis.

Pathways

CD98 is a vital component of the mTOR signaling pathway influencing cell growth and proliferation. It also plays a part in the integrin signaling pathway which regulates cell adhesion and migration. In these pathways CD98 works closely with proteins like FG1 and mTORC1. These relationships enable it to modulate cellular responses to nutritional availability and mechanical cues from the environment.

CD98 expression and function have links to cancer and autoimmune diseases. High expression levels of CD98 are observed in various cancers such as lymphomas where it correlates with increased tumor growth and poor prognosis. In autoimmune disorders like rheumatoid arthritis CD98 influences immune cell activation and migration. Proteins such as CP1 and FG1 interact with CD98 contributing to disease pathology by affecting cellular and immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters (PubMed : 10574970, PubMed : 10903140, PubMed : 11557028, PubMed : 30867591, PubMed : 33298890, PubMed : 33758168, PubMed : 34880232, PubMed : 9751058, PubMed : 9829974, PubMed : 9878049). Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids (PubMed : 10903140, PubMed : 9829974). Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed : 34880232). SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane (By similarity). SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA (PubMed : 10574970, PubMed : 11389679, PubMed : 11557028, PubMed : 11564694, PubMed : 11742812, PubMed : 12117417, PubMed : 12225859, PubMed : 12716892, PubMed : 15980244, PubMed : 30867591, PubMed : 33298890, PubMed : 33758168). SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8 (PubMed : 10391915, PubMed : 10574970, PubMed : 11311135, PubMed : 15769744, PubMed : 33066406). When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed : 25998567). Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression (PubMed : 11121428, PubMed : 15625115).. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis.. (Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells.
See full target information SLC3A2
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