Rabbit Recombinant Monoclonal CDK4 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (AB193967), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cdk4-antibody-epr4513-32-7-ab193967--immunocytochemistry-immunofluorescence-img55584.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (AB193967)")
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (AB193967), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cdk4-antibody-epr4513-32-7-ab193967--immunocytochemistry-immunofluorescence-img128178.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (AB193967)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Not recommended |
| Rat | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Species Rat | Dilution info - | Notes This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
Associated Products
Select an associated product type
7 products for Alternative Product
5 products for Alternative Version
Target data
Function
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Alternative names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
Recommended products
Rabbit Recombinant Monoclonal CDK4 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.
Alternative names
Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR4513-32-7
- Purification technique
Affinity purification Protein A
- Dissociation constant
1.86 x 10-11 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cdk4-antibody-epr4513-32-7-ab193967--immunocytochemistry-immunofluorescence-img55584.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967)
ab193967 staining Cdk4 in wild-type HAP1 cells (top panel) and Cdk4 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab193967 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cdk4-antibody-epr4513-32-7-ab193967--immunocytochemistry-immunofluorescence-img128178.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cdk4 antibody [EPR4513-32-7] (ab193967)
ab193967 staining Cdk4 in T47D cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab193967 at a 1/500 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
Downloads
Product protocols
- Visit the general protocols
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