AB199819

Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal p16INK4A antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CDKN2, MTS1, CDKN2A, Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, CDK4I, MTS-1, p16-INK4, p16INK4A, MTS2, CDKN2B, Cyclin-dependent kinase 4 inhibitor B, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b, MTS-2, p15INK4B

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (AB199819)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (AB199819)

ab199819 staining CDKN2A/p16INK4a in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199819 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 2μg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R]
Unconjugated

Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R]
Carrier free

Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP435Y-129R

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the gene CDKN2A acts as a negative regulator of normal cell proliferation by strongly interacting with CDK4 and CDK6. This interaction inhibits the ability of CDK4 and CDK6 to interact with cyclins D and to phosphorylate the retinoblastoma protein. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CDKN2A

Additional targets

CDKN2B

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 27:234 PubMed38596263

2024

Circulating tumor cells help differentiate benign ovarian lesions from cancer before surgery: A literature review and proof of concept study using flow cytometry with fluorescence imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Yung-Chia Kuo,Chi-Hsi Chuang,Hsuan-Chih Kuo,Cheng-Tao Lin,Angel Chao,Huei-Jean Huang,Hung-Ming Wang,Jason Chia-Hsun Hsieh,Hung-Hsueh Chou

Clinical and translational medicine 13:e1518 PubMed38148658

2023

Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dai,Sijie Wang,Xinggang Guo,Yuefan Wang,Haozan Yin,Jian Tan,Chenyang Mu,Shu-Han Sun,Hui Liu,Fu Yang

Journal of personalized medicine 11: PubMed34834510

2021

Circulating p16-Positive and p16-Negative Tumor Cells Serve as Independent Prognostic Indicators of Survival in Patients with Head and Neck Squamous Cell Carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Pei-Hung Chang,Hung-Ming Wang,Yung-Chia Kuo,Li-Yu Lee,Chia-Jung Liao,Hsuan-Chih Kuo,Cheng-Lung Hsu,Chun-Ta Liao,Sanger Hung-Chi Lin,Pei-Wei Huang,Tyler Min-Hsien Wu,Jason Chia-Hsun Hsieh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDKN2A/p16INK4A + CDKN2B/p15INK4B antibody [EP435Y-129R] (AB199819)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (3)

Product promise