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Rabbit Recombinant Monoclonal CDT1/DUP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDT1/DUP antibody [EPR17891] (AB211857), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)

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Target data

Function

Required for both DNA replication and mitosis (PubMed:11125146, PubMed:14993212, PubMed:21856198, PubMed:22581055, PubMed:26842564). DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC) (PubMed:14672932). Required also for mitosis by promoting stable kinetochore-microtubule attachments (PubMed:22581055). Potential oncogene (By similarity).

Alternative names

DNA replication factor Cdt1, Double parked homolog, DUP, CDT1

Recommended products

Rabbit Recombinant Monoclonal CDT1/DUP antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 647
Excitation/Emission
Ex: 650nm, Em: 665nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17891
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CDT1 also known as DUP or CDT-1 is an important regulator in cell cycle control. CDT1 has a molecular weight of approximately 68 kDa and is expressed predominantly in the nucleus during the late M and early G1 phases of the cell cycle. This protein plays an essential role in the licensing of DNA replication origins ensuring that DNA replication occurs precisely once per cell cycle. The careful regulation of CDT1 activity is necessary to prevent re-replication which can lead to genomic instability.

Biological function summary

This protein is necessary for the correct assembly of the pre-replicative complex (pre-RC) on replication origins. CDT1 works closely with CDC6 and the minichromosome maintenance (MCM) complex to form the pre-RC facilitating the loading of MCM helicase onto the DNA. This process tightly regulates replication origin licensing and ensures DNA replication fidelity. Misregulation of CDT1 can disrupt cell cycle progression and compromise genomic integrity.

Pathways

CDT1 functions primarily in cell cycle regulation and DNA replication. It participates in the origin recognition complex (ORC) pathway that initiates DNA replication. The proper function of CDT1 is critical to these pathways working alongside other proteins such as ORC1 and MCM complex members. Together they contribute to the accurate duplication of the genome which is pivotal for cell proliferation and maintenance of chromosomal stability.

Associated diseases and disorders

CDT1 dysregulation associates with cancer and genomic instability. Overexpression or uncontrolled activity of CDT1 can lead to re-replication increasing the risk of mutations and chromosomal aberrations characteristic of neoplastic transformations. The protein's relationship with MCM components particularly those involved in cancer-related pathways highlights its relevance in tumorigenesis. Understanding CDT1's role in these diseases guides the development of targeted therapies and diagnostic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDT1/DUP antibody [EPR17891] (ab211857), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CDT1/DUP antibody [EPR17891] (ab211857)

    ab211857 staining CDT1/DUP in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211857 at 1/50 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com