AB196860

Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Citrate synthetase antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Citrate (Si)-synthase, CS

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067] (AB196860)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067] (AB196860)

ab196860 staining Citrate synthetase in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196860 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 488, shown in green) at 1/167 dilution, overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067] (AB196860)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067] (AB196860)

Overlay histogram showing HeLa cells stained with ab196860 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab196860, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 647 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Citrate synthetase antibody [EPR8067]
HRP

HRP Anti-Citrate synthetase antibody [EPR8067]
660 APC

APC Anti-Citrate synthetase antibody [EPR8067]
578 PE

PE Anti-Citrate synthetase antibody [EPR8067]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Citrate synthetase antibody [EPR8067]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Citrate synthetase antibody [EPR8067]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Citrate synthetase antibody [EPR8067]
Unconjugated

Anti-Citrate synthetase antibody [EPR8067]
Carrier free

Anti-Citrate synthetase antibody [EPR8067] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8067

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor®; 647), is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.

Biological function summary

Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.

Pathways

Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.

Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.

See full target information Citrate synthase, mitochondrial

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 118:105848 PubMed40645008

2025

Genotype and transcript processing of the tumour necrosis factor receptor TNFRSF1A in epithelial cells: implications for survival in cystic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Uden,Inga Dunsche,Sabina Janciauskiene,Simon Gräber,Longhua Feng,Stephanie Tamm,Silke Hedtfeld,Gesa Stege,Kirsten Jahn,Julia Kontsendorn,Nadine Alfeis,Iris Kühbandner,Rebecca Minso,Christian Dopfer,Matthias Griese,Olaf Sommerburg,Felix C Ringshausen,Lutz Nährlich,Gesine Hansen,Tobias Welte,Peter Braubach,Marcus A Mall,Burkhard Tümmler,Anna-Maria Dittrich,Frauke Stanke

Proceedings of the National Academy of Sciences of the United States of America 121:e2404841121 PubMed39348545

2024

TCA metabolism regulates DNA hypermethylation in LPS and -induced immune tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Abhimanyu,Santiago Carrero Longlax,Tomoki Nishiguchi,Malik Ladki,Daanish Sheikh,Amera L Martinez,Emily M Mace,Sandra L Grimm,Thaleia Caldwell,Alexandra Portillo Varela,Rajagopal V Sekhar,Anna M Mandalakas,Mandla Mlotshwa,Sibuse Ginidza,Jeffrey D Cirillo,Robert S Wallis,Mihai G Netea,Reinout van Crevel,Cristian Coarfa,Andrew R DiNardo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067] (AB196860)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Citrate synthetase antibody [EPR8067] (AB196860)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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