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AB319619

Alexa Fluor® 647 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

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Rabbit Recombinant Monoclonal Claudin 18 antibody - conjugated to Alexa Fluor® 647.

View Alternative Names

UNQ778/PRO1572, CLDN18, Claudin-18

  • 660 APC

    APC Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 578 PE

    PE Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Claudin18.2 + Claudin18.1 antibody [EPR19203]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19203

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

Target Binding Affinity, Antibody Labelling

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Claudin18.2 and Claudin18.1 are integral membrane proteins part of the claudin family which are tight junction proteins. Claudin18.2 has a molecular weight of approximately 27 kDa. These proteins are primarily expressed in epithelial cells particularly within the gastric mucosa and lung tissue. They serve as essential components of cell-cell adhesion influencing paracellular transport and maintaining cell polarity.
Biological function summary

These proteins are vital in forming tight junction strands contributing to cellular barrier functions. Claudin18.2 and Claudin18.1 belong to a multi-protein complex vital for establishing cell permeability limits. Through their integration in tight junctions they regulate ion passage minimize fluid loss and maintain tissue homeostasis. Their dynamic nature allows adaptation in response to physiological stimuli making them central to epithelial barrier integrity.

Pathways

Claudins including Claudin18.2 and Claudin18.1 play roles in signaling pathways like Wnt and MAPK which are pivotal for cellular processes like proliferation and differentiation. These pathways involve interactions with proteins like ZO-1 and occludin contributing to the signaling processes across the epithelial cells. This involvement with important signaling proteins highlights their importance in maintaining cell function and signaling responses.

These claudins have strong associations with gastric cancer and asthma pathophysiology. Overexpression of Claudin18.2 has been shown in gastric cancer indicating its potential as a therapeutic target. In asthma altered expression of Claudin18.1 affects lung epithelial integrity linking these proteins to airway inflammation and remodeling. The connection with proteins like E-cadherin and beta-catenin further highlights their role in tumor progression and epithelial transformation making them relevant in clinical research.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

The protein expressed by the gene CLDN18 plays a major role in the tight junction-specific obliteration of the intercellular space through calcium-independent cell-adhesion activity. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CLDN18

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com