Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785]

3 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (ab280968)

  Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling Collagen I with ab280968 at 1/100 dilution.
  Positive staining on the stroma in human endometrium. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab280968 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (ab280968)

  Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Collagen I with ab280968 at 1/100 dilution.
  Negative control: no staining on the human cardiac muscle.
  

  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab280968 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Collagen I antibody [EPR7785] (ab280968)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Collagen I with ab280968 at 1/100 dilution.
  Negative control: no staining on the human cerebrum.
  

  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab280968 at 1/100 dilution (shown in Red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).