Rabbit Recombinant Monoclonal COUP TF1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (AB210038), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-coup-tf1-antibody-epr10841-ab210038--immunocytochemistry-immunofluorescence-img94613.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (AB210038)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
3 products for Alternative Version
Target data
Function
Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
Alternative names
EAR3, ERBAL3, TFCOUP1, NR2F1, COUP transcription factor 1, COUP-TF1, COUP transcription factor I, Nuclear receptor subfamily 2 group F member 1, V-erbA-related protein 3, COUP-TF I, EAR-3
Recommended products
Rabbit Recombinant Monoclonal COUP TF1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR10841
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
COUP TF1 also known as COUP-TFI COUP-TF1 COUPF protein COUP 70 and counter COUP functions as a transcription factor. It belongs to the nuclear receptor family of proteins and has a molecular mass of approximately 47 kDa. This protein expresses in a variety of tissues including the central nervous system heart and developing organs. It plays a role in regulating gene expression through binding to DNA sequences ultimately controlling developmental processes and cellular differentiation.
- Biological function summary
COUP TF1 influences numerous systems by regulating genes involved in neuronal development and organogenesis. This protein is a core component of transcription complexes interacting with other nuclear receptors and co-regulators to modulate its effects. The activity of COUP TF1 also extends to the regulation of neuronal subtype specification and synaptic connectivity signifying its importance in the central nervous system development.
- Pathways
COUP TF1 participates in essential signaling cascades such as the Notch and retinoic acid pathways. It modulates the retinoic acid response by interacting with the retinoic acid receptor (RAR) thereby influencing cell differentiation and growth. Additionally COUP TF1 interacts with the Notch signaling pathway affecting neural lineage decisions and maintenance. Its link to other transcription factors and receptors highlights its integrative role in various cellular contexts.
- Associated diseases and disorders
COUP TF1 alterations associate with cognitive and developmental disorders. Its dysregulation is implicated in conditions like mental retardation and schizophrenia. The protein's connection to disorders often involves its interaction with other proteins and signaling pathways such as those linked to neural development. COUP TF1's interplay with the retinoic acid receptor influences neurological traits and misregulation can contribute to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-coup-tf1-antibody-epr10841-ab210038--immunocytochemistry-immunofluorescence-img94613.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)
ab210038 staining COUP TF1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210038 at 1/100 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
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