Rabbit Recombinant Monoclonal CPSF6 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CPSF6 antibody [EPR12898] (AB225247), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cpsf6-antibody-epr12898-ab225247--immunocytochemistry-immunofluorescence-img41380.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CPSF6 antibody [EPR12898] (AB225247)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chicken | Predicted |
| Cow | Predicted |
| Xenopus laevis | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow, Xenopus laevis | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
4 products for Alternative Version
Target data
Function
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed:15169763, PubMed:29276085, PubMed:21295486). Plays a role in mRNA export (PubMed:19864460).(Microbial infection) Binds HIV-1 capsid-nucleocapsid (HIV-1 CA-NC) complexes and might thereby promote the integration of the virus in the nucleus of dividing cells (in vitro).
Alternative names
Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF 68 kDa subunit, CFIm68, CPSF6, CFIM68
Recommended products
Rabbit Recombinant Monoclonal CPSF6 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples.
Alternative names
Cleavage and polyadenylation specificity factor subunit 6, Cleavage and polyadenylation specificity factor 68 kDa subunit, Cleavage factor Im complex 68 kDa subunit, Pre-mRNA cleavage factor Im 68 kDa subunit, Protein HPBRII-4/7, CPSF 68 kDa subunit, CFIm68, CPSF6, CFIM68
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 647
- Excitation/Emission
Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR12898
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- Activity summary
Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) also referred to as CFIm68 for its molecular weight of approximately 68 kDa plays an important role in mRNA processing. The protein is expressed widely in various tissues emphasizing its broad functional role in cellular mechanisms. CPSF6 operates as an important component of the cleavage and polyadenylation complex where it facilitates the regulation of pre-mRNA 3'-end processing influencing gene expression control.
- Biological function summary
CPSF6 influences alternative polyadenylation impacting mRNA maturation by recognizing specific RNA sequences. As part of a broader complex this protein engages in cooperation with components like CPSF5 and CPSF7 showcasing its integrated role in this essential process. The regulation exerted by CPSF6 on polyadenylation modifies the length and stability of mRNA transcripts. This impact on mRNA dynamics is important for normal cellular function and response.
- Pathways
CPSF6 integrates into key mRNA processing and maturation pathways affecting transcriptional regulation and mRNA stability. This protein functionally interlinks with RNA polymerase II playing an integral part in the transcriptional machinery. CPSF6 also associates with the spliceosome complex a critical group in mRNA splicing establishing its multi-functional role within gene expression pathways.
- Associated diseases and disorders
CPSF6 associations emerge prominently in cancer and viral infections. Abnormalities in CPSF6 expression or function can contribute to oncogenic processes through dysregulated mRNA processing. Additionally CPSF6 has significant links to HIV where it interacts with viral components affecting virus replication. The relationship between CPSF6 and other proteins like CPSF1 in these contexts highlights its involvement in disease progression mechanisms rendering it a potential target for therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CPSF6 antibody [EPR12898] (ab225247), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cpsf6-antibody-epr12898-ab225247--immunocytochemistry-immunofluorescence-img41380.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CPSF6 antibody [EPR12898] (ab225247)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CPSF6 antibody [EPR12898] (ab225247)
ab225247 staining CPSF6 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225247 at 1/1000 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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