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AB204666

Alexa Fluor® 647 Anti-CRMP1 + CRMP2 antibody [EPR7792]

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(1 Publication)

Rabbit Recombinant Monoclonal CRMP2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

View Alternative Names

CRMP2, ULIP2, DPYSL2, Dihydropyrimidinase-related protein 2, DRP-2, Collapsin response mediator protein 2, N2A3, Unc-33-like phosphoprotein 2, CRMP-2, ULIP-2, DPYSL1, ULIP3, CRMP1, Dihydropyrimidinase-related protein 1, DRP-1, Collapsin response mediator protein 1, Inactive dihydropyrimidinase, Unc-33-like phosphoprotein 3, CRMP-1, ULIP-3

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB204666)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CRMP1 + CRMP2 antibody [EPR7792] (AB204666)

ab204666 staining CRMP2 in PC12 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204666 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in PC12 cells fixed with 100% methanol (5min).

  • Carrier free

    Anti-CRMP1 + CRMP2 antibody [EPR7792] - BSA and Azide free

  • Unconjugated

    Anti-CRMP1 + CRMP2 antibody [EPR7792]

  • 660 APC

    APC Anti-CRMP2 antibody [EPR7792]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CRMP2 antibody [EPR7792]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CRMP2 antibody [EPR7792]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CRMP1 + CRMP2 antibody [EPR7792]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CRMP2 antibody [EPR7792]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CRMP1 + CRMP2 antibody [EPR7792]

  • HRP

    HRP Anti-CRMP2 antibody [EPR7792]

  • 578 PE

    PE Anti-CRMP2 antibody [EPR7792]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7792

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB62478

Anti-CRMP2 (phospho T514) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T514) antibody (AB62478)

  • 0
  • 0
Primary Antibodies

AB192799

Anti-CRMP2 (phospho T509) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T509) antibody (AB192799)

  • 0
  • 0
Primary Antibodies

AB36201

Anti-CRMP2 antibody

Immunohistochemistry (Frozen sections) - Anti-CRMP2 antibody (AB36201)

  • 0
  • 0
Primary Antibodies

AB129066

Anti-CRMP2 (phospho T514) antibody [EPR7876]

RabMAb|Recombinant

Western blot - Anti-CRMP2 (phospho T514) antibody [EPR7876] (AB129066)

  • 0
  • 0
Primary Antibodies

AB215742

Anti-CRMP2 (phospho T555) antibody [M539]

Western blot - Anti-CRMP2 (phospho T555) antibody [M539] (AB215742)

  • 0
  • 0
Primary Antibodies

AB62539

Anti-CRMP2 antibody [1B1]

KO Validated

Flow Cytometry - Anti-CRMP2 antibody [1B1] (AB62539)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB97180

Goat Anti-Rat IgM mu chain (HRP)

  • 5
  • 1
Proteins & Peptides

AB114269

Recombinant Human CRMP2 protein (GST tag N-Terminus)

Western blot - Recombinant Human CRMP2 protein (GST tag N-Terminus) (AB114269)

  • 0
  • 0
Secondary Antibodies

AB191866

VHH Single Domain Anti-Rabbit IgG Fc (HRP)

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (AB191866)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in PC12 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.
See full target information DPYSL2

Additional targets

CRMP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:1031278 PubMed36518186

2022

Cochlear resident macrophage mediates development of ribbon synapses CX3CR1/CX3CL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Song,Yang Li,Rui Guo,Qianru Yu,Shan Liu,Qi Teng,Zhong-Rui Chen,Jing Xie,Shusheng Gong,Ke Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com