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AB204663

Alexa Fluor® 647 Anti-CTBP2 antibody [EPR7611(B)]

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(5 Publications)

Rabbit Recombinant Monoclonal CTBP2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

C-terminal-binding protein 2, CtBP2, CTBP2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CTBP2 antibody [EPR7611(B)] (AB204663)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CTBP2 antibody [EPR7611(B)] (AB204663)

ab204663 staining CTBP2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204663 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Unconjugated

    Anti-CTBP2 antibody [EPR7611(B)]

  • 660 APC

    APC Anti-CTBP2 antibody [EPR7611(B)]

  • HRP

    HRP Anti-CTBP2 antibody [EPR7611(B)]

  • 578 PE

    PE Anti-CTBP2 antibody [EPR7611(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CTBP2 antibody [EPR7611(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CTBP2 antibody [EPR7611(B)]

  • Carrier free

    Anti-CTBP2 antibody [EPR7611(B)] - BSA and Azide free

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CTBP2 antibody [EPR7611(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CTBP2 antibody [EPR7611(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CTBP2 antibody [EPR7611(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7611(B)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CTBP2 also known as C-terminal binding protein 2 is a transcriptional co-repressor involved in regulating gene expression. It is a protein with a molecular mass of approximately 48 kDa. CTBP2 is expressed in various tissues with high levels in neural muscular and epithelial cells. Mechanically CTBP2 operates by interacting with transcription factors and histone-modifying enzymes modulating chromatin structure to control access to DNA.
Biological function summary

CTBP2 plays a significant role in cellular processes such as development and differentiation. It frequently acts as part of multi-protein complexes interacting with diverse partners to facilitate transcriptional repression. These interactions are essential for balancing gene activity ensuring appropriate tissue development and function. CTBP2 influences cellular dynamics by regulating target genes implicated in growth survival and metabolic pathways.

Pathways

CTBP2 integrates into significant signaling pathways like Wnt and TGF-beta where it reinforces regulatory control by interacting with critical factors such as beta-catenin or Smad proteins. It collaborates with other proteins like CTBP1 dictating cellular responses and influencing pathophysiological processes. By coordinating these pathways CTBP2 supports cellular homeostasis impacting tissues in response to external and internal cues.

CTBP2 has associations with cancer and neurodegenerative diseases. Its overexpression or altered activity links to cancer progression as it promotes a transcriptional environment conducive to tumor growth. Proteins such as E-cadherin connect with CTBP2 in metastasis exemplifying its role in cancer cell dissemination. Additionally changes in CTBP2 function relate to neurodegenerative conditions affecting nerve cell survival and synaptic activity pointing to its involvement in neuronal health and disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity).. Isoform 2 probably acts as a scaffold for specialized synapses.
See full target information CTBP2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:1031278 PubMed36518186

2022

Cochlear resident macrophage mediates development of ribbon synapses CX3CR1/CX3CL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Song,Yang Li,Rui Guo,Qianru Yu,Shan Liu,Qi Teng,Zhong-Rui Chen,Jing Xie,Shusheng Gong,Ke Liu

Frontiers in molecular neuroscience 15:850035 PubMed35310883

2022

Autophagy-Mediated Synaptic Refinement and Auditory Neural Pruning Contribute to Ribbon Synaptic Maturity in the Developing Cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Guo,Yice Xu,Wei Xiong,Wei Wei,Yue Qi,Zhengde Du,Shusheng Gong,Zezhang Tao,Ke Liu

Neural plasticity 2022:5567174 PubMed35096052

2022

ROS-Induced Oxidative Damage and Mitochondrial Dysfunction Mediated by Inhibition of SIRT3 in Cultured Cochlear Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lingjun Zhang,Zhengde Du,Lu He,Wenqi Liang,Ke Liu,Shusheng Gong

American journal of translational research 12:7354-7366 PubMed33312372

2020

Loss of cochlear ribbon synapses in the early stage of aging causes initial hearing impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xiong,Shukui Yu,Ke Liu,Shusheng Gong

Neuroscience 406:345-355 PubMed30742960

2019

Long-Term Conductive Auditory Deprivation During Early Development Causes Irreversible Hearing Impairment and Cochlear Synaptic Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Qi,Shukui Yu,Zhengde Du,Tengfei Qu,Lu He,Wei Xiong,Wei Wei,Ke Liu,Shusheng Gong
View all publications
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