AB194231

Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 10 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

KPP, KRT10, Cytokeratin-10, Keratin-10, CK-10, K10

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (AB194231)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (AB194231)

ab194231 staining Cytokeratin 10 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194231 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

This product also gave a positive signal in 4% formaldehyde (10min) fixed HeLa cells under the same testing conditions.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

HRP

HRP Anti-Cytokeratin 10 antibody [EP1607IHCY]
Unconjugated

Anti-Cytokeratin 10 antibody [EP1607IHCY] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 10 antibody [EP1607IHCY] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1607IHCY

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "Signal can be observed in cells fixed with MeOH or PFA.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark|Store at -80°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 10 also known as CK10 K10 or KRT10 is a type I keratin protein with a mass of about 59 kDa. Researchers recognize it as an important structural protein found mainly in the suprabasal layers of the epidermis where it contributes to maintaining the integrity and function of the skin barrier. Cytokeratin 10 is also expressed in other stratified squamous epithelia. Scientists use antibodies against cytokeratin 10 sometimes called 'lh2' and 'cytokeratin 10 anticuerpos' as neomarkers for identifying specific cell types in tissue studies.

Biological function summary

Keratin proteins like cytokeratin 10 provide mechanical stability to cells and tissues. Cytokeratin 10 forms a heterodimer with keratin 1 (KRT1) creating a complex that plays a critical role in the cytoskeleton of keratinocytes. This structure supports cellular resilience against mechanical stresses helping maintain the integrity of epithelial tissues. Keratin 10 function is essential for normal skin development and differentiation highlighting its significance in epidermal morphogenesis.

Pathways

Cytokeratin 10 is integral to the epidermal differentiation complex. This pathway involves many regulatory proteins and is essential for proper skin formation and repair. Cytokeratin 10 together with KRT1 contributes to the terminal differentiation of keratinocytes. Another key pathway is the cornification process where cytokeratin 10 helps form the protective outer layer of the skin. These biological roles place cytokeratin 10 at an essential point in maintaining skin homeostasis and function.

Abnormal expression or mutation of cytokeratin 10 leads to skin conditions like epidermolytic hyperkeratosis. This rare inherited disorder results in blistering and thickening of the skin. Cytokeratin 10 mutations often appear alongside changes in KRT1 due to their forming a functional pair indicating their collaborative role in disease manifestation. Additionally altered cytokeratin 10 expression can be associated with psoriasis as disrupted keratinocyte differentiation contributes to this inflammatory skin disease. Understanding these associations helps in studying and developing treatment strategies for skin disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity).. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.

See full target information KRT10

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 221: PubMed39352576

2024

Incontinentia pigmenti underlies thymic dysplasia, autoantibodies to type I IFNs, and viral diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Jérémie Rosain,Tom Le Voyer,Xian Liu,Adrian Gervais,Laura Polivka,Axel Cederholm,Laureline Berteloot,Audrey V Parent,Alessandra Pescatore,Ezia Spinosa,Snezana Minic,Ana Elisa Kiszewski,Miyuki Tsumura,Chloé Thibault,Maria Esnaola Azcoiti,Jelena Martinovic,Quentin Philippot,Taushif Khan,Astrid Marchal,Bénédicte Charmeteau-De Muylder,Lucy Bizien,Caroline Deswarte,Lillia Hadjem,Marie-Odile Fauvarque,Karim Dorgham,Daniel Eriksson,Emilia Liana Falcone,Mathilde Puel,Sinem Ünal,Amyrath Geraldo,Corentin Le Floc'h,Hailun Li,Sylvie Rheault,Christine Muti,Claire Bobrie-Moyrand,Anne Welfringer-Morin,Ramsay L Fuleihan,Romain Lévy,Marie Roelens,Liwei Gao,Marie Materna,Silvia Pellegrini,Lorenzo Piemonti,Emilie Catherinot,Jean-Christophe Goffard,Arnaud Fekkar,Aissata Sacko-Sow,Camille Soudée,Soraya Boucherit,Anna-Lena Neehus,Cristina Has,Stefanie Hübner,Géraldine Blanchard-Rohner,Blanca Amador-Borrero,Takanori Utsumi,Maki Taniguchi,Hiroo Tani,Kazushi Izawa,Takahiro Yasumi,Sotaro Kanai,Mélanie Migaud,Mélodie Aubart,Nathalie Lambert,Guy Gorochov,Capucine Picard,Claire Soudais,Anne-Sophie L'Honneur,Flore Rozenberg,Joshua D Milner,Shen-Ying Zhang,Pierre Vabres,Dusan Trpinac,Nico Marr,Nathalie Boddaert,Isabelle Desguerre,Manolis Pasparakis,Corey N Miller,Cláudia S Poziomczyk,Laurent Abel,Satoshi Okada,Emmanuelle Jouanguy,Rémi Cheynier,Qian Zhang,Aurélie Cobat,Vivien Béziat,Bertrand Boisson,Julie Steffann,Francesca Fusco,Matilde Valeria Ursini,Smail Hadj-Rabia,Christine Bodemer,Jacinta Bustamante,Hervé Luche,Anne Puel,Gilles Courtois,Paul Bastard,Nils Landegren,Mark S Anderson,Jean-Laurent Casanova

Nature 623:803-813 PubMed37938781

2023

Autoantibodies against type I IFNs in humans with alternative NF-κB pathway deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Le Voyer,Audrey V Parent,Xian Liu,Axel Cederholm,Adrian Gervais,Jérémie Rosain,Tina Nguyen,Malena Perez Lorenzo,Elze Rackaityte,Darawan Rinchai,Peng Zhang,Lucy Bizien,Gonca Hancioglu,Pascale Ghillani-Dalbin,Jean-Luc Charuel,Quentin Philippot,Mame Sokhna Gueye,Majistor Raj Luxman Maglorius Renkilaraj,Masato Ogishi,Camille Soudée,Mélanie Migaud,Flore Rozenberg,Mana Momenilandi,Quentin Riller,Luisa Imberti,Ottavia M Delmonte,Gabriele Müller,Baerbel Keller,Julio Orrego,William Alexander Franco Gallego,Tamar Rubin,Melike Emiroglu,Nima Parvaneh,Daniel Eriksson,Maribel Aranda-Guillen,David I Berrios,Linda Vong,Constance H Katelaris,Peter Mustillo,Johannes Raedler,Jonathan Bohlen,Jale Bengi Celik,Camila Astudillo,Sarah Winter,Catriona McLean,Aurélien Guffroy,Joseph L DeRisi,David Yu,Corey Miller,Yi Feng,Audrey Guichard,Vivien Béziat,Jacinta Bustamante,Qiang Pan-Hammarström,Yu Zhang,Lindsey B Rosen,Steve M Holland,Marita Bosticardo,Heather Kenney,Riccardo Castagnoli,Charlotte A Slade,Kaan Boztuğ,Nizar Mahlaoui,Sylvain Latour,Roshini S Abraham,Vassilios Lougaris,Fabian Hauck,Anna Sediva,Faranaz Atschekzei,Georgios Sogkas,M Cecilia Poli,Mary A Slatter,Boaz Palterer,Michael D Keller,Alberto Pinzon-Charry,Anna Sullivan,Luke Droney,Daniel Suan,Melanie Wong,Alisa Kane,Hannah Hu,Cindy Ma,Hana Grombiříková,Peter Ciznar,Ilan Dalal,Nathalie Aladjidi,Miguel Hie,Estibaliz Lazaro,Jose Franco,Sevgi Keles,Marion Malphettes,Marlene Pasquet,Maria Elena Maccari,Andrea Meinhardt,Aydan Ikinciogullari,Mohammad Shahrooei,Fatih Celmeli,Patrick Frosk,Christopher C Goodnow,Paul E Gray,Alexandre Belot,Hye Sun Kuehn,Sergio D Rosenzweig,Makoto Miyara,Francesco Licciardi,Amélie Servettaz,Vincent Barlogis,Guillaume Le Guenno,Vera-Maria Herrmann,Taco Kuijpers,Grégoire Ducoux,Françoise Sarrot-Reynauld,Catharina Schuetz,Charlotte Cunningham-Rundles,Frédéric Rieux-Laucat,Stuart G Tangye,Cristina Sobacchi,Rainer Doffinger,Klaus Warnatz,Bodo Grimbacher,Claire Fieschi,Laureline Berteloot,Vanessa L Bryant,Sophie Trouillet Assant,Helen Su,Benedicte Neven,Laurent Abel,Qian Zhang,Bertrand Boisson,Aurélie Cobat,Emmanuelle Jouanguy,Olle Kampe,Paul Bastard,Chaim M Roifman,Nils Landegren,Luigi D Notarangelo,Mark S Anderson,Jean-Laurent Casanova,Anne Puel

Nature communications 13:5481 PubMed36123328

2022

Modeling human HSV infection via a vascularized immune-competent skin-on-chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Sijie Sun,Lei Jin,Ying Zheng,Jia Zhu

Aging 12:12002-12018 PubMed32570219

2020

Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wei,Aixue Wang,Qing Wang,Wenrui Han,Rong Rong,Lijuan Wang,Sijia Liu,Yimeng Zhang,Chao Dong,Yanling Li

Materials science & engineering. C, Materials for biological applications 103:109759 PubMed31349449

2019

Transition metal-doped cryogels as bioactive materials for wound healing applications.

Applications

Unspecified application

Species

Unspecified reactive species

Duong Luong,Abdulla A Yergeshov,Mohamed Zoughaib,Farida R Sadykova,Bulat I Gareev,Irina N Savina,Timur I Abdullin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (AB194231)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise