Rabbit Recombinant Monoclonal Cytokeratin 10 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (AB194231), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cytokeratin-10-antibody-ep1607ihcy-ab194231--immunocytochemistry-immunofluorescence-img40937.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (AB194231)")
Publications
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- Nature 623:803-8132023Autoantibodies against type I IFNs in humans with alternative NF-κB pathway deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTom Le Voyer,Audrey V Parent,Xian Liu,Axel Cederholm,Adrian Gervais,Jérémie Rosain,Tina Nguyen,Malena Perez Lorenzo,Elze Rackaityte,Darawan Rinchai,Peng Zhang,Lucy Bizien,Gonca Hancioglu,Pascale Ghillani-Dalbin,Jean-Luc Charuel,Quentin Philippot,Mame Sokhna Gueye,Majistor Raj Luxman Maglorius Renkilaraj,Masato Ogishi,Camille Soudée,Mélanie Migaud,Flore Rozenberg,Mana Momenilandi,Quentin Riller,Luisa Imberti,Ottavia M Delmonte,Gabriele Müller,Baerbel Keller,Julio Orrego,William Alexander Franco Gallego,Tamar Rubin,Melike Emiroglu,Nima Parvaneh,Daniel Eriksson,Maribel Aranda-Guillen,David I Berrios,Linda Vong,Constance H Katelaris,Peter Mustillo,Johannes Raedler,Jonathan Bohlen,Jale Bengi Celik,Camila Astudillo,Sarah Winter,Catriona McLean,Aurélien Guffroy,Joseph L DeRisi,David Yu,Corey Miller,Yi Feng,Audrey Guichard,Vivien Béziat,Jacinta Bustamante,Qiang Pan-Hammarström,Yu Zhang,Lindsey B Rosen,Steve M Holland,Marita Bosticardo,Heather Kenney,Riccardo Castagnoli,Charlotte A Slade,Kaan Boztuğ,Nizar Mahlaoui,Sylvain Latour,Roshini S Abraham,Vassilios Lougaris,Fabian Hauck,Anna Sediva,Faranaz Atschekzei,Georgios Sogkas,M Cecilia Poli,Mary A Slatter,Boaz Palterer,Michael D Keller,Alberto Pinzon-Charry,Anna Sullivan,Luke Droney,Daniel Suan,Melanie Wong,Alisa Kane,Hannah Hu,Cindy Ma,Hana Grombiříková,Peter Ciznar,Ilan Dalal,Nathalie Aladjidi,Miguel Hie,Estibaliz Lazaro,Jose Franco,Sevgi Keles,Marion Malphettes,Marlene Pasquet,Maria Elena Maccari,Andrea Meinhardt,Aydan Ikinciogullari,Mohammad Shahrooei,Fatih Celmeli,Patrick Frosk,Christopher C Goodnow,Paul E Gray,Alexandre Belot,Hye Sun Kuehn,Sergio D Rosenzweig,Makoto Miyara,Francesco Licciardi,Amélie Servettaz,Vincent Barlogis,Guillaume Le Guenno,Vera-Maria Herrmann,Taco Kuijpers,Grégoire Ducoux,Françoise Sarrot-Reynauld,Catharina Schuetz,Charlotte Cunningham-Rundles,Frédéric Rieux-Laucat,Stuart G Tangye,Cristina Sobacchi,Rainer Doffinger,Klaus Warnatz,Bodo Grimbacher,Claire Fieschi,Laureline Berteloot,Vanessa L Bryant,Sophie Trouillet Assant,Helen Su,Benedicte Neven,Laurent Abel,Qian Zhang,Bertrand Boisson,Aurélie Cobat,Emmanuelle Jouanguy,Olle Kampe,Paul Bastard,Chaim M Roifman,Nils Landegren,Luigi D Notarangelo,Mark S Anderson,Jean-Laurent Casanova,Anne PuelPubMed 37938781
- Aging 12:12002-120182020Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Wei et. al.PubMed 32570219
- Materials science & engineering. C, Materials for biological applications 103:1097592019Transition metal-doped cryogels as bioactive materials for wound healing applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuong Luong,Abdulla A Yergeshov,Mohamed Zoughaib,Farida R Sadykova,Bulat I Gareev,Irina N Savina,Timur I AbdullinPubMed 31349449
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes Signal can be observed in cells fixed with MeOH or PFA. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
2 products for Alternative Version
Target data
Function
Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity). (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
Alternative names
KPP, KRT10, Cytokeratin-10, Keratin-10, CK-10, K10
Recommended products
Rabbit Recombinant Monoclonal Cytokeratin 10 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP1607IHCY
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C, Store in the dark, Store at -80°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cytokeratin 10 also known as CK10 K10 or KRT10 is a type I keratin protein with a mass of about 59 kDa. Researchers recognize it as an important structural protein found mainly in the suprabasal layers of the epidermis where it contributes to maintaining the integrity and function of the skin barrier. Cytokeratin 10 is also expressed in other stratified squamous epithelia. Scientists use antibodies against cytokeratin 10 sometimes called 'lh2' and 'cytokeratin 10 anticuerpos' as neomarkers for identifying specific cell types in tissue studies.
- Biological function summary
Keratin proteins like cytokeratin 10 provide mechanical stability to cells and tissues. Cytokeratin 10 forms a heterodimer with keratin 1 (KRT1) creating a complex that plays a critical role in the cytoskeleton of keratinocytes. This structure supports cellular resilience against mechanical stresses helping maintain the integrity of epithelial tissues. Keratin 10 function is essential for normal skin development and differentiation highlighting its significance in epidermal morphogenesis.
- Pathways
Cytokeratin 10 is integral to the epidermal differentiation complex. This pathway involves many regulatory proteins and is essential for proper skin formation and repair. Cytokeratin 10 together with KRT1 contributes to the terminal differentiation of keratinocytes. Another key pathway is the cornification process where cytokeratin 10 helps form the protective outer layer of the skin. These biological roles place cytokeratin 10 at an essential point in maintaining skin homeostasis and function.
- Associated diseases and disorders
Abnormal expression or mutation of cytokeratin 10 leads to skin conditions like epidermolytic hyperkeratosis. This rare inherited disorder results in blistering and thickening of the skin. Cytokeratin 10 mutations often appear alongside changes in KRT1 due to their forming a functional pair indicating their collaborative role in disease manifestation. Additionally altered cytokeratin 10 expression can be associated with psoriasis as disrupted keratinocyte differentiation contributes to this inflammatory skin disease. Understanding these associations helps in studying and developing treatment strategies for skin disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab194231), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-647-cytokeratin-10-antibody-ep1607ihcy-ab194231--immunocytochemistry-immunofluorescence-img40937.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab194231)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 10 antibody [EP1607IHCY] (ab194231)
ab194231 staining Cytokeratin 10 in A431 cells. The cells were fixed with 100% methanol (5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194231 at a working dilution of 1 in 50 (shown in red) and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
This product also gave a positive signal in 4% formaldehyde (10min) fixed HeLa cells under the same testing conditions.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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