AB206100

Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350]

Name: Anti-Cytokeratin 14 Alexa Fluor® 647 antibody [EPR17350] (ab206100) | Abcam
Brand: Abcam Limited
SKU: AB206100
Price: 620 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 14 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

2 Images

Lab

Immunocytochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

ab206100 staining Cytokeratin 14 in A431 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206100 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in A431 cells fixed with 100% methanol (5 min).

PubMed

Immunocytochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Immunocytochemistry/immunofluorescence analysis of human mammary organoids grown on chamber slides labelling Cytokeratin 8 with ab192467 (green), Cytokeratin 14 with ab206100 (red), and Actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 μm. Organoids were imaged by confocal microscopy.

Rosenbluth J et al., Nat Commun, 11(1), 1711. Fig 1c.; doi: 10.1038/s41467-020-15548-7. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cytokeratin 14 antibody [EPR17350]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EPR17350]
Unconjugated

Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17350

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p>This product gave a positive signal in A431 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

See full target information Keratin, type I cytoskeletal 14

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1470379 PubMed39469640

2024

Modeling epithelial-mesenchymal transition in patient-derived breast cancer organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Neta Bar-Hai,Rakefet Ben-Yishay,Sheli Arbili-Yarhi,Naama Herman,Vered Avidan-Noy,Tehillah Menes,Aiham Mansour,Fahim Awwad,Nora Balint-Lahat,Gil Goldinger,Goni Hout-Siloni,Mohammad Adileh,Raanan Berger,Dana Ishay-Ronen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309220 PubMed39023197

2024

A Linkable, Polycarbonate Gut Microbiome-Distal Tumor Chip Platform for Interrogating Cancer Promoting Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle S K Brasino,Sean D Speese,Kevin Schilling,Carolyn E Schutt,Michelle C Barton

Cell reports 42:113293 PubMed37858468

2023

Single-cell and spatial analyses reveal a tradeoff between murine mammary proliferation and lineage programs associated with endocrine cues.

Applications

Unspecified application

Species

Unspecified reactive species

G Kenneth Gray,Nomeda Girnius,Hendrik J Kuiken,Aylin Z Henstridge,Joan S Brugge

Cell reports 33:108566 PubMed33378681

2020

Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Carman Man-Chung Li,Hana Shapiro,Christina Tsiobikas,Laura M Selfors,Huidong Chen,Jennifer Rosenbluth,Kaitlin Moore,Kushali P Gupta,G Kenneth Gray,Yaara Oren,Michael J Steinbaugh,Jennifer L Guerriero,Luca Pinello,Aviv Regev,Joan S Brugge

Nature communications 11:1711 PubMed32249764

2020

Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages.

Applications

ICC

Species

Unspecified reactive species

Jennifer M Rosenbluth,Ron C J Schackmann,G Kenneth Gray,Laura M Selfors,Carman Man-Chung Li,Mackenzie Boedicker,Hendrik J Kuiken,Andrea Richardson,Jane Brock,Judy Garber,Deborah Dillon,Norman Sachs,Hans Clevers,Joan S Brugge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350]

Immunocytochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Immunocytochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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