Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

Immunohistochemical analysis of Paraffin-embedded Human gastric cancer tissue labelling Cytokeratin 19 with ab192980 at 1/100 dilution.

Membrane staining on human gastric cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

Immunohistochemical analysis of Paraffin-embedded Human breast cancer tissue labelling Cytokeratin 19 with ab192980 at 1/100 dilution.

Membrane staining on human breast cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

Immunohistochemical analysis of Paraffin-embedded Human glioma tissue labelling Cytokeratin 19 with ab192980 at 1/100 dilution.

Negative control : no staining on human glioma.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

Immunohistochemical analysis of Paraffin-embedded Human lung cancer tissue labelling Cytokeratin 19 with ab192980 at 1/100 dilution.

Membrane staining on human lung cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labelling Cytokeratin 19 with ab192980 at 1/100 dilution.

Membrane staining on human pancreas.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

ab192980 staining Cytokeratin 19 in HeLa cells. The cells were fixed with 100% methanol (5 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192980 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with an AlexaFluor^® 488 Goat anti-mouse IgG (H&L - preadsorbed) secondary (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

This product gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions

Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

Overlay histogram showing HeLa cells stained with ab192980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192980, 0.2μl/1x10⁶ cells) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Alexa Fluor^® 647) (0.1μg/1x10⁶ cells) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 25mW red solid state diode laser (635nm) and 675/30 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192980)

ab192980 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192980 at 1/100 dilution (shown in red) and ab195887 (Mouse monoclonal to alpha Tubulin - Alexa Fluor® 488) at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).