AB192468

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]

Name: Alexa Fluor® 647 conjugated Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468) | Abcam
Brand: Abcam Limited
SKU: AB192468
Price: 560 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(14 Publications)

Anti-Cytokeratin 8 antibody [EP1628Y] - Alexa Fluor® 647 conjugated (ab192468) is a rabbit recombinant monoclonal antibody detecting Cytokeratin 8 in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

ab192468 staining Cytokeratin 8 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192468 at a working dilution of 1 in 50 (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor^® 488 Goat anti-mouse IgG (H&L - preadsorbed) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells tested under the same conditions.

Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

Overlay histogram showing HeLa cells stained with ab192468 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192468, 2μl/1x10⁶) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (Alexa Fluor^® 647) (2μl/1x10⁶) for 30 min at 22°C. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 25mW red solid state diode laser (635nm) and 675/30 bandpass filter.

This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

578 PE

PE Anti-Cytokeratin 8 antibody [EP1628Y]
421 Alexa Fluor® 405

Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
HRP

HRP Anti-Cytokeratin 8 antibody [EP1628Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1628Y

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µL for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/alexa-fluor-647-rabbit-igg-monoclonal-epr25a-isotype-control-ab199093'>ab199093</a> - Rabbit monoclonal IgG (Alexa Fluor® 647), is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP1628Y is also available pre-conjugated to a variety of labels for your convenience – Anti-Cytokeratin 8 Alexa Fluor® 647 [EP1628Y] (ab192468).

Other related products
We have a range of other formats of antibody clone [EP1628Y] also available for your convenience: ab53280, Alexa Fluor® 488 - ab192467, Alexa Fluor® 647 - ab192468, HRP - ab193094, PE - ab209297, Alexa Fluor® 405 - ab210139, Carrier free - ab217173, Carrier free - ab302461

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

The Journal of experimental medicine 221: PubMed39352576

2024

Incontinentia pigmenti underlies thymic dysplasia, autoantibodies to type I IFNs, and viral diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Jérémie Rosain,Tom Le Voyer,Xian Liu,Adrian Gervais,Laura Polivka,Axel Cederholm,Laureline Berteloot,Audrey V Parent,Alessandra Pescatore,Ezia Spinosa,Snezana Minic,Ana Elisa Kiszewski,Miyuki Tsumura,Chloé Thibault,Maria Esnaola Azcoiti,Jelena Martinovic,Quentin Philippot,Taushif Khan,Astrid Marchal,Bénédicte Charmeteau-De Muylder,Lucy Bizien,Caroline Deswarte,Lillia Hadjem,Marie-Odile Fauvarque,Karim Dorgham,Daniel Eriksson,Emilia Liana Falcone,Mathilde Puel,Sinem Ünal,Amyrath Geraldo,Corentin Le Floc'h,Hailun Li,Sylvie Rheault,Christine Muti,Claire Bobrie-Moyrand,Anne Welfringer-Morin,Ramsay L Fuleihan,Romain Lévy,Marie Roelens,Liwei Gao,Marie Materna,Silvia Pellegrini,Lorenzo Piemonti,Emilie Catherinot,Jean-Christophe Goffard,Arnaud Fekkar,Aissata Sacko-Sow,Camille Soudée,Soraya Boucherit,Anna-Lena Neehus,Cristina Has,Stefanie Hübner,Géraldine Blanchard-Rohner,Blanca Amador-Borrero,Takanori Utsumi,Maki Taniguchi,Hiroo Tani,Kazushi Izawa,Takahiro Yasumi,Sotaro Kanai,Mélanie Migaud,Mélodie Aubart,Nathalie Lambert,Guy Gorochov,Capucine Picard,Claire Soudais,Anne-Sophie L'Honneur,Flore Rozenberg,Joshua D Milner,Shen-Ying Zhang,Pierre Vabres,Dusan Trpinac,Nico Marr,Nathalie Boddaert,Isabelle Desguerre,Manolis Pasparakis,Corey N Miller,Cláudia S Poziomczyk,Laurent Abel,Satoshi Okada,Emmanuelle Jouanguy,Rémi Cheynier,Qian Zhang,Aurélie Cobat,Vivien Béziat,Bertrand Boisson,Julie Steffann,Francesca Fusco,Matilde Valeria Ursini,Smail Hadj-Rabia,Christine Bodemer,Jacinta Bustamante,Hervé Luche,Anne Puel,Gilles Courtois,Paul Bastard,Nils Landegren,Mark S Anderson,Jean-Laurent Casanova

iScience 27:110668 PubMed39246442

2024

Multiplex imaging of localized prostate tumors reveals altered spatial organization of AR-positive cells in the microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Çiğdem Ak,Zeynep Sayar,Guillaume Thibault,Erik A Burlingame,M J Kuykendall,Jennifer Eng,Alex Chitsazan,Koei Chin,Andrew C Adey,Christopher Boniface,Paul T Spellman,George V Thomas,Ryan P Kopp,Emek Demir,Young Hwan Chang,Vasilis Stavrinides,Sebnem Ece Eksi

Nature 627:656-663 PubMed38418883

2024

An atlas of epithelial cell states and plasticity in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guangchun Han,Ansam Sinjab,Zahraa Rahal,Anne M Lynch,Warapen Treekitkarnmongkol,Yuejiang Liu,Alejandra G Serrano,Jiping Feng,Ke Liang,Khaja Khan,Wei Lu,Sharia D Hernandez,Yunhe Liu,Xuanye Cao,Enyu Dai,Guangsheng Pei,Jian Hu,Camille Abaya,Lorena I Gomez-Bolanos,Fuduan Peng,Minyue Chen,Edwin R Parra,Tina Cascone,Boris Sepesi,Seyed Javad Moghaddam,Paul Scheet,Marcelo V Negrao,John V Heymach,Mingyao Li,Steven M Dubinett,Christopher S Stevenson,Avrum E Spira,Junya Fujimoto,Luisa M Solis,Ignacio I Wistuba,Jichao Chen,Linghua Wang,Humam Kadara

Nature 623:803-813 PubMed37938781

2023

Autoantibodies against type I IFNs in humans with alternative NF-κB pathway deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Tom Le Voyer,Audrey V Parent,Xian Liu,Axel Cederholm,Adrian Gervais,Jérémie Rosain,Tina Nguyen,Malena Perez Lorenzo,Elze Rackaityte,Darawan Rinchai,Peng Zhang,Lucy Bizien,Gonca Hancioglu,Pascale Ghillani-Dalbin,Jean-Luc Charuel,Quentin Philippot,Mame Sokhna Gueye,Majistor Raj Luxman Maglorius Renkilaraj,Masato Ogishi,Camille Soudée,Mélanie Migaud,Flore Rozenberg,Mana Momenilandi,Quentin Riller,Luisa Imberti,Ottavia M Delmonte,Gabriele Müller,Baerbel Keller,Julio Orrego,William Alexander Franco Gallego,Tamar Rubin,Melike Emiroglu,Nima Parvaneh,Daniel Eriksson,Maribel Aranda-Guillen,David I Berrios,Linda Vong,Constance H Katelaris,Peter Mustillo,Johannes Raedler,Jonathan Bohlen,Jale Bengi Celik,Camila Astudillo,Sarah Winter,Catriona McLean,Aurélien Guffroy,Joseph L DeRisi,David Yu,Corey Miller,Yi Feng,Audrey Guichard,Vivien Béziat,Jacinta Bustamante,Qiang Pan-Hammarström,Yu Zhang,Lindsey B Rosen,Steve M Holland,Marita Bosticardo,Heather Kenney,Riccardo Castagnoli,Charlotte A Slade,Kaan Boztuğ,Nizar Mahlaoui,Sylvain Latour,Roshini S Abraham,Vassilios Lougaris,Fabian Hauck,Anna Sediva,Faranaz Atschekzei,Georgios Sogkas,M Cecilia Poli,Mary A Slatter,Boaz Palterer,Michael D Keller,Alberto Pinzon-Charry,Anna Sullivan,Luke Droney,Daniel Suan,Melanie Wong,Alisa Kane,Hannah Hu,Cindy Ma,Hana Grombiříková,Peter Ciznar,Ilan Dalal,Nathalie Aladjidi,Miguel Hie,Estibaliz Lazaro,Jose Franco,Sevgi Keles,Marion Malphettes,Marlene Pasquet,Maria Elena Maccari,Andrea Meinhardt,Aydan Ikinciogullari,Mohammad Shahrooei,Fatih Celmeli,Patrick Frosk,Christopher C Goodnow,Paul E Gray,Alexandre Belot,Hye Sun Kuehn,Sergio D Rosenzweig,Makoto Miyara,Francesco Licciardi,Amélie Servettaz,Vincent Barlogis,Guillaume Le Guenno,Vera-Maria Herrmann,Taco Kuijpers,Grégoire Ducoux,Françoise Sarrot-Reynauld,Catharina Schuetz,Charlotte Cunningham-Rundles,Frédéric Rieux-Laucat,Stuart G Tangye,Cristina Sobacchi,Rainer Doffinger,Klaus Warnatz,Bodo Grimbacher,Claire Fieschi,Laureline Berteloot,Vanessa L Bryant,Sophie Trouillet Assant,Helen Su,Benedicte Neven,Laurent Abel,Qian Zhang,Bertrand Boisson,Aurélie Cobat,Emmanuelle Jouanguy,Olle Kampe,Paul Bastard,Chaim M Roifman,Nils Landegren,Luigi D Notarangelo,Mark S Anderson,Jean-Laurent Casanova,Anne Puel

Cell reports 42:113293 PubMed37858468

2023

Single-cell and spatial analyses reveal a tradeoff between murine mammary proliferation and lineage programs associated with endocrine cues.

Applications

Unspecified application

Species

Unspecified reactive species

G Kenneth Gray,Nomeda Girnius,Hendrik J Kuiken,Aylin Z Henstridge,Joan S Brugge

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23176 PubMed37665592

2023

A genome-wide transposon mutagenesis screening identifies LppB as a key factor associated with Mycoplasma bovis colonization and invasion into host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shimei Lan,Zhangcheng Li,Huafang Hao,Shuang Liu,Zhicheng Huang,Yutong Bai,Yanzhao Li,Xinmin Yan,Pengcheng Gao,Shengli Chen,Yuefeng Chu

Breast cancer research : BCR 25:23 PubMed36859337

2023

Lineage plasticity enables low-ER luminal tumors to evolve and gain basal-like traits.

Applications

Unspecified application

Species

Unspecified reactive species

Gadisti Aisha Mohamed,Sundis Mahmood,Nevena B Ognjenovic,Min Kyung Lee,Owen M Wilkins,Brock C Christensen,Kristen E Muller,Diwakar R Pattabiraman

Nature cancer 3:1071-1087 PubMed36065066

2022

Ectopic JAK-STAT activation enables the transition to a stem-like and multilineage state conferring AR-targeted therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Su Deng,Choushi Wang,Yunguan Wang,Yaru Xu,Xiaoling Li,Nickolas A Johnson,Atreyi Mukherji,U-Ging Lo,Lingfan Xu,Julisa Gonzalez,Lauren A Metang,Jianfeng Ye,Carla Rodriguez Tirado,Kathia Rodarte,Yinglu Zhou,Zhiqun Xie,Carlos Arana,Valli Annamalai,Xihui Liu,Donald J Vander Griend,Douglas Strand,Jer-Tsong Hsieh,Bo Li,Ganesh Raj,Tao Wang,Ping Mu

Nature communications 12:7292 PubMed34911933

2021

Epigenetic loss of heterogeneity from low to high grade localized prostate tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Sebnem Ece Eksi,Alex Chitsazan,Zeynep Sayar,George V Thomas,Andrew J Fields,Ryan P Kopp,Paul T Spellman,Andrew C Adey

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

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