JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB199543

Alexa Fluor® 647 Anti-DcR1 antibody [EPR6162]

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal DcR1 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD263, DCR1, LIT, TRAILR3, TRID, UNQ321/PRO366, TNFRSF10C, Tumor necrosis factor receptor superfamily member 10C, Antagonist decoy receptor for TRAIL/Apo-2L, Decoy TRAIL receptor without death domain, Decoy receptor 1, Lymphocyte inhibitor of TRAIL, TNF-related apoptosis-inducing ligand receptor 3, TRAIL receptor without an intracellular domain, DcR1, TRAIL receptor 3, TRAIL-R3

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DcR1 antibody [EPR6162] (AB199543)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DcR1 antibody [EPR6162] (AB199543)

ab199543 staining DcR1 in Jurkat cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199543 at 1/50 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6162

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

This antibody was developed as part of a collaboration with the lab of Shi-Yong Sun at Emory University.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for the cytotoxic ligand TRAIL. Lacks a cytoplasmic death domain and hence is not capable of inducing apoptosis. May protect cells against TRAIL mediated apoptosis by competing with TRAIL-R1 and R2 for binding to the ligand.
See full target information TNFRSF10C
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 46:101996 PubMed38795560

2024

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingke Li,Zhiwu Wang,Jian Wang,Jiangong Wang,Xuan Zheng,Dan Li,Zhuo Wang,Jingwu Li,Yufeng Li
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB243087

APC Anti-DcR1 antibody [TRAIL-R3-02]

Flow Cytometry - APC Anti-DcR1 antibody [TRAIL-R3-02] (AB243087)

  • 0
  • 0
Primary Antibodies

AB2087

Anti-DcR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DcR1 antibody (AB2087)

  • 0
  • 0

Complementary Products

Assay Kits

AB314710

Human DcR1 R3 ELISA Kit

Sandwich ELISA - Human DcR1 R3 ELISA Kit (AB314710)

  • 0
  • 0
Proteins & Peptides

AB276266

Recombinant human DcR1 protein (His tag)

Functional Studies - Recombinant human DcR1 protein (His tag) (AB276266)

  • 0
  • 0
Primary Antibodies

AB243076

Alexa Fluor® 647 Anti-DcR2 antibody [TRAIL-R4-01]

Flow Cytometry - Alexa Fluor® 647 Anti-DcR2 antibody [TRAIL-R4-01] (AB243076)

  • 0
  • 0
Primary Antibodies

AB181036

Anti-EAAT1 antibody [EPR12686]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (AB181036)

  • 5
  • 3
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com