Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
- RabMAb
- Recombinant
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(4 Publications)
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - Alexa Fluor® 647 conjugated (ab245893) is a rabbit recombinant monoclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in ICC/IF.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245893)
ab245893 staining DDDDK tag in 293T cells transfected with a FLAG®-tagged PFKFB3 expression vector. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245893 at 1/50 dilution (shown in red) and a Mouse monoclonal anti-FLAG® M2 antibody used as a counterstain. This was followed by an incubation at room temperature for 1h with ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Related conjugates and formulations (2)
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
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HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
Reactivity data
Product details
What is this antibody validated in?
Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.
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Related products
Antibody clone EPR20018-251 is also available pre-conjugated to a variety of labels for your convenience – Anti-DDDDK tag (Binds to FLAG® tag sequence) Alexa Fluor® 647 [EPR20018-251] (ab245893).
Other related products
We have a range of other formats of antibody clone [EPR20018-251] also available for your convenience: ab205606, Carrier free - ab236777, Alexa Fluor® 488 - ab245892, Alexa Fluor® 647 - ab245893, HRP - ab245895
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Publications (4)
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Frontiers in immunology 15:1468258 PubMed39539545
2024
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Nature communications 15:7553 PubMed39215044
2024
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Cell discovery 9:105 PubMed37875488
2023
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Bioengineering & translational medicine 8:e10482 PubMed38023709
2023
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Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com