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AB245893

Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

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(4 Publications)

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - Alexa Fluor® 647 conjugated (ab245893) is a rabbit recombinant monoclonal antibody detecting DDDDK tag (Binds to FLAG® tag sequence) in ICC/IF.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

DDDDK epitope tag, DDDK, DYKDDDDK, DYKDDDDK (also known as the FLAG搴 tag sequence), DYKDDDDK epitope tag, DYKDDDDK tag, ECS epitope tag, ECS tag, Enterokinase Cleavage Site epitope tag, Enterokinase Cleavage Site tag, FLAG搴, FLAG搴 tag, ddk

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245893)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245893)

ab245893 staining DDDDK tag in 293T cells transfected with a FLAG®-tagged PFKFB3 expression vector. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245893 at 1/50 dilution (shown in red) and a Mouse monoclonal anti-FLAG® M2 antibody used as a counterstain. This was followed by an incubation at room temperature for 1h with ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

  • HRP

    HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20018-251

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in FLAG®-tagged PFKFB3 expression construct transfected 293T cells fixed with 4% formaldehyde (10 min).</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

Product details

What is this antibody validated in?
Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (ab245893) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
DDDDK tag (Binds to FLAG® tag sequence) antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR20018-251 is also available pre-conjugated to a variety of labels for your convenience – Anti-DDDDK tag (Binds to FLAG® tag sequence) Alexa Fluor® 647 [EPR20018-251] (ab245893).

Other related products
We have a range of other formats of antibody clone [EPR20018-251] also available for your convenience: ab205606, Carrier free - ab236777, Alexa Fluor® 488 - ab245892, Alexa Fluor® 647 - ab245893, HRP - ab245895

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1468258 PubMed39539545

2024

CRISPR/Cas9-mediated knockout of STAT1 in porcine-derived cell lines to elucidate the role of STAT1 in autophagy following classical swine fever virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Zhang,Dongli Liang,Kaijun Min,Jiaxin Liang,Yu Tian,Cheng Liu,Ting Rong Luo,Xiaoning Li

Nature communications 15:7553 PubMed39215044

2024

Tumor-agnostic cancer therapy using antibodies targeting oncofetal chondroitin sulfate.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Ethel Vidal-Calvo,Anne Martin-Salazar,Swati Choudhary,Robert Dagil,Sai Sundar Rajan Raghavan,Lara Duvnjak,Mie Anemone Nordmaj,Thomas Mandel Clausen,Ann Skafte,Jan Oberkofler,Kaituo Wang,Mette Ø Agerbæk,Caroline Løppke,Amalie Mundt Jørgensen,Daria Ropac,Joana Mujollari,Shona Willis,Agnès Garcias López,Rebecca Louise Miller,Richard Torbjörn Gustav Karlsson,Felix Goerdeler,Yen-Hsi Chen,Ana R Colaço,Yong Wang,Thomas Lavstsen,Agnieszka Martowicz,Irina Nelepcu,Mona Marzban,Htoo Zarni Oo,Maj Sofie Ørum-Madsen,Yuzhuo Wang,Morten A Nielsen,Henrik Clausen,Michael Wierer,Dominik Wolf,Ismail Gögenur,Thor G Theander,Nader Al-Nakouzi,Tobias Gustavsson,Mads Daugaard,Ali Salanti

Cell discovery 9:105 PubMed37875488

2023

Ectopic expression of human TUBB8 leads to increased aneuploidy in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Dong,Liping Jin,Shihua Bao,Biaobang Chen,Yang Zeng,Yuxi Luo,Xingzhu Du,Qing Sang,Tianyu Wu,Lei Wang

Bioengineering & translational medicine 8:e10482 PubMed38023709

2023

Genetically engineered nano-melittin vesicles for multimodal synergetic cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhong Zhang,Xue Liu,Yutian Xia,Shuyu Xu,Xuan Liu,Haiqing Xiao,Xiaoyong Wang,Chao Liu,Gang Liu
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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