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AB225252

Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal DDX39 antibody - conjugated to Alexa Fluor® 647. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DDX39, DDX39A, ATP-dependent RNA helicase DDX39A, DEAD box protein 39, Nuclear RNA helicase URH49

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)] (AB225252)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)] (AB225252)

ab225252 staining DDX39 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225252 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)] (AB225252)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)] (AB225252)

Overlay histogram showing HeLa cells stained with ab225252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225252, 1/50000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.

This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Carrier free

    Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free

  • Unconjugated

    Anti-DDX39 antibody [EPR13507(B)]

  • 578 PE

    PE Anti-DDX39 antibody [EPR13507(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13507(B)

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DDX39 protein also known as DDX39A or BAT1 belongs to the DEAD box family of proteins and has a molecular mass of approximately 49 kDa. Mechanically DDX39 functions as an RNA helicase which plays a critical role in the unwinding of RNA secondary structures during various RNA metabolic processes. The protein facilitates RNA splicing transport and translation by interacting with these fundamental cellular mechanisms. DDX39 is widely expressed in various tissues including liver heart and brain highlighting its importance across different biological systems.
Biological function summary

The multifunctional attributes of DDX39 contribute significantly to RNA processing events. The protein serves as an integral part of the TREX (transcription/export) complex which mediates the export of mRNA from the nucleus to the cytoplasm. By unwinding RNA DDX39 ensures proper mRNA maturation and transport essential for accurate gene expression. The involvement of DDX39 in the TREX complex highlights its central role in maintaining cellular homeostasis.

Pathways

DDX39 participates actively in gene expression and mRNA processing pathways. The protein's role in the TREX complex links it to the mRNA export pathway important for synthesizing proteins and cell cycle progression. Through its activities DDX39 interacts with related proteins such as ALYREF and UAP56 coordinating the transfer of mRNA. These interactions highlight its collaborative function within complex cellular processes.

DDX39 has connections to cancer including breast and lung cancer where altered mRNA processing contributes to tumor progression. The protein associates closely with other mRNA processing components like ALYREF where dysregulated interactions might influence cancer cell proliferation. Additionally DDX39 abnormalities have associations with spinal muscular atrophy where its involvement in RNA processing affects motor neuron health. This links DDX39 through its complex roles in both cancer and neurodegenerative disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Helicase that plays an essential role in mRNA export and is involved in multiple steps in RNA metabolism including alternative splicing (PubMed : 33941617, PubMed : 38801080). Regulates nuclear mRNA export to the cytoplasm through association with ECD (PubMed : 33941617). Also involved in spliceosomal uridine-rich small nuclear RNA (U snRNA) export by stimulating the RNA binding of adapter PHAX (PubMed : 39011894). Plays a role in the negative regulation of type I IFN production by increasing the nuclear retention of antiviral transcripts and thus reducing their protein expression (PubMed : 32393512). Independently of the interferon pathway, plays an antiviral role against alphaviruses by binding to a 5' conserved sequence element in the viral genomic RNA (PubMed : 37949067).
See full target information DDX39A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40565279

2025

Molecular Determinants of the Human Retinal Pigment Epithelium Cell Fate and Potential Pharmacogenomic Targets for Precision Medicine.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Zibetti
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com