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AB319130

Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706]

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Rabbit Recombinant Monoclonal delta Sarcoglycan antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

Delta-sarcoglycan, Delta-SG, 35 kDa dystrophin-associated glycoprotein, 35DAG, SGCD

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706] (AB319130)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706] (AB319130)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling delta Sarcoglycan with ab319130 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human colon.
The section was incubated with ab319130 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706] (AB319130)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706] (AB319130)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling delta Sarcoglycan with ab319130 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human skeletal muscle.
The section was incubated with ab319130 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706] (AB319130)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-delta Sarcoglycan antibody [EPR8706] (AB319130)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling delta Sarcoglycan with ab319130 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human cardiac muscle.
The section was incubated with ab319130 for 60 mins at room temperature (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Unconjugated

    Anti-delta Sarcoglycan antibody [EPR8706]

  • Carrier free

    Anti-delta Sarcoglycan antibody [EPR8706] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8706

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Proteins & Peptides

AB140566

Recombinant Human delta Sarcoglycan protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human delta Sarcoglycan protein (denatured) (His tag N-Terminus) (AB140566)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta Sarcoglycan also known as SGCD is a protein component of the sarcoglycan complex. It has an approximate mass of 35 kDa. Delta Sarcoglycan localizes predominantly in skeletal and cardiac muscle tissues. This protein forms part of a set of transmembrane glycoproteins which are integral to the muscle cell membrane. It plays an important role in maintaining the structural integrity of muscle cells by linking the sarcolemma to the extracellular matrix.
Biological function summary

Delta Sarcoglycan associates with other sarcoglycan proteins to form the sarcoglycan complex. This complex is an essential part of the dystrophin-associated glycoprotein complex (DAGC) which connects the cytoskeleton of a muscle fiber to the extracellular matrix. The proper function of this complex is essential for muscle stability during contraction and relaxation preventing damage to the muscle fibers. It interacts with dystrophin an essential adapter protein which anchors the sarcolemma to the cytoskeleton.

Pathways

Delta Sarcoglycan plays an integral role in maintaining the integrity of the dystrophin-glycoprotein complex pathway which is vital for muscle function. It also involves the adhesion and signaling pathways that influence muscle cell adhesion and communication. It shows a relationship with dystrophin where mutations in genes coding for any component including delta Sarcoglycan disrupt these pathways and affect muscle tissue integrity.

Delta Sarcoglycan mutations are primarily linked to limb-girdle muscular dystrophy type 2F (LGMD2F) and specific types of cardiomyopathy. These conditions manifest through progressive muscle weakness and deterioration emphasizing the protein’s significance in muscle health. Mutations in the dystrophin protein can exacerbate these diseases further illustrating the important synergy between dystrophin and sarcoglycan complex including delta Sarcoglycan in maintaining muscle integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
See full target information Delta-sarcoglycan
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com