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AB322025

Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768]

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Rabbit Recombinant Monoclonal Desmoglein 2/DSG2 antibody - conjugated to Alexa Fluor® 647. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB322025)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB322025)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Desmoglein 2/DSG2 with ab322025 at 1/100 (5.0 ug/ml) dilution.

Low expression tissue : no staining on human liver.
The section was incubated with ab322025 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB322025)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB322025)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Desmoglein 2/DSG2 with ab322025 at 1/100 (5.0 ug/ml) dilution.

Membranous staining on human lung cancer.
The section was incubated with ab322025 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB322025)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB322025)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Desmoglein 2/DSG2 with ab322025 at 1/100 (5.0 ug/ml) dilution.

Membranous staining on human breast.
The section was incubated with ab322025 for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Desmoglein 2/DSG2 antibody [EPR6768]

  • Unconjugated

    Anti-Desmoglein 2/DSG2 antibody [EPR6768]

  • Carrier free

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free

  • Carrier free

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6768

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB314727

Human Desmoglein 2/DSG2 ELISA Kit

Sandwich ELISA - Human Desmoglein 2/DSG2 ELISA Kit (AB314727)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.
Biological function summary

DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.

Pathways

DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.

DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 17559062, PubMed : 38395410). Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Required for proliferation and viability of embryonic stem cells in the blastocyst, thereby crucial for progression of post-implantation embryonic development (By similarity). Maintains pluripotency by regulating epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via interacting with and sequestering CTNNB1 to sites of cell-cell contact, thereby reducing translocation of CTNNB1 to the nucleus and subsequent transcription of CTNNB1/TCF-target genes (PubMed : 29910125). Promotes pluripotency and the multi-lineage differentiation potential of hematopoietic stem cells (PubMed : 27338829). Plays a role in endothelial cell sprouting and elongation via mediating the junctional-association of cortical actin fibers and CDH5 (PubMed : 27338829). Promotes cardiomyocyte cell homeostasis and desmosome junction formation at intercalated disks, as a result plays a role in the maintenance of cardiac conduction and heart chamber integrity (By similarity). Positively regulates pancreatic islet development and maintenance of endothelial cell barrier integrity in the pancreas, therefore involved in the controlled release of insulin from islet cells into the circulation in response to glucose (By similarity). Plays a role in limiting inflammatory infiltration and the apoptotic response to injury in kidney tubular epithelial cells, potentially via its role in maintaining cell-cell adhesion and the epithelial barrier (PubMed : 38395410). Acts as a positive modulator of CSK and EGFR activation via sequestering them away from lipid rafts, this is independent of its role in desmosome cell junctions (PubMed : 26918609). Also disrupts the localization of CAV1 to lipid rafts resulting in its distribution throughout the cytoplasm (PubMed : 26918609).
See full target information DSG2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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