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AB314815

Alexa Fluor® 647 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

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Rabbit Recombinant Monoclonal Dnmt3a mutated R882H antibody - conjugated to Alexa Fluor® 647. Suitable for ICC/IF and reacts with Transfected cell line - Human samples.

View Alternative Names

DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, Cysteine methyltransferase DNMT3A, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, M.HsaIIIA, DNMT3A

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11] (AB314815)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11] (AB314815)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Dnmt3a with ab314815 at 1/50 (10.0 ug/ml) dilution. Confocal showing positive staining in HEK-293T cells transfected with a human DNMT3A (R882H) expression vector containing a myc tag, and showing no staining in HEK-293 cells transfected with human wildtype DNMT3A expression vector containing a myc tag. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 0.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

  • Unconjugated

    Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

  • 578 PE

    PE Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

  • Carrier free

    Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11] - BSA and Azide free

  • 660 APC

    APC Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Dnmt3a (mutated R882H) antibody [EPR27389-11]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27389-11

Isotype

IgG

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). DNA methylation is coordinated with methylation of histones (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed : 12138111, PubMed : 16357870, PubMed : 30478443). May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (By similarity). Plays a role in paternal and maternal imprinting (By similarity). Required for methylation of most imprinted loci in germ cells (By similarity). Acts as a transcriptional corepressor for ZBTB18 (By similarity). Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity). Can actively repress transcription through the recruitment of HDAC activity (By similarity). Also has weak auto-methylation activity on Cys-710 in absence of DNA (By similarity).
See full target information DNMT3A mutated R882H
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com